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| Status |
Public on Mar 23, 2022 |
| Title |
Transcriptome profiling of Arabidopsis roots upon lateral root induction in WT and TOR knock down [dataset1] |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To get a genome wide picture of the effects of TOR knockdown during the early phase of lateral root formation, we compared by RNA-seq the transcriptomes of roots 6h after the synchronous induction of lateral formation by auxin treatment (Himanen et al, 2002 - http://dx.doi.org/10.1105/tpc.004960) in the inducible UB10pro>>amiR-TOR background.
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| Overall design |
Samples (Col-0 or inducible UB10pro>>amiR-TOR line) were prepared for harvesting using the synchronous induction of lateral root procedure. All samples were pre-treated with 10 µM NPA for 24 h then shifted to plates containing 10 µM NPA and 10 µM Estradiol or DMSO control for additional 24 h before being shifted to either 10 µM IAA + 10 µM Estradiol or DMSO for LR induction or maintained on the same plates. Root tissue was harvested after 6 h. All sampling points were performed in triplicate. For each sample, about 200 segments of the lower two-thirds of the seedling roots were pooled.
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| Contributor(s) |
Maizel A |
| Citation missing |
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| Submission date |
Mar 23, 2022 |
| Last update date |
Mar 26, 2022 |
| Contact name |
Alexis Maizel |
| E-mail(s) |
alexis.maizel@cos.uni-heidelberg.de
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| Organization name |
Heidelberg University
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| Department |
Center for Organismal Studies
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| Street address |
Im Neuenheimer Feld 230
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| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
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| Platforms (1) |
| GPL19580 |
Illumina NextSeq 500 (Arabidopsis thaliana) |
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| Samples (24)
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| This SubSeries is part of SuperSeries: |
| GSE199211 |
TOR acts as metabolic gatekeeper for auxin-dependent lateral root initiation in Arabidopsis thaliana |
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| Relations |
| BioProject |
PRJNA818879 |
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