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Series GSE199202 Query DataSets for GSE199202
Status Public on Mar 23, 2022
Title Transcriptome profiling of Arabidopsis roots upon lateral root induction in WT and TOR knock down [dataset1]
Organism Arabidopsis thaliana
Experiment type Expression profiling by high throughput sequencing
Summary To get a genome wide picture of the effects of TOR knockdown during the early phase of lateral root formation, we compared by RNA-seq the transcriptomes of roots 6h after the synchronous induction of lateral formation by auxin treatment (Himanen et al, 2002 - in the inducible UB10pro>>amiR-TOR background.
Overall design Samples (Col-0 or inducible UB10pro>>amiR-TOR line) were prepared for harvesting using the synchronous induction of lateral root procedure. All samples were pre-treated with 10 µM NPA for 24 h then shifted to plates containing 10 µM NPA and 10 µM Estradiol or DMSO control for additional 24 h before being shifted to either 10 µM IAA + 10 µM Estradiol or DMSO for LR induction or maintained on the same plates. Root tissue was harvested after 6 h. All sampling points were performed in triplicate. For each sample, about 200 segments of the lower two-thirds of the seedling roots were pooled.
Contributor(s) Maizel A
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Submission date Mar 23, 2022
Last update date Mar 26, 2022
Contact name Alexis Maizel
Organization name Heidelberg University
Department Center for Organismal Studies
Street address Im Neuenheimer Feld 230
City Heidelberg
ZIP/Postal code 69120
Country Germany
Platforms (1)
GPL19580 Illumina NextSeq 500 (Arabidopsis thaliana)
Samples (24)
GSM5966625 Col-0_NPA_Es_1
GSM5966626 UB10pro_amiR-TOR_NPA_1
GSM5966627 UB10pro_amiR-TOR_NPA_Es_1
This SubSeries is part of SuperSeries:
GSE199211 TOR acts as metabolic gatekeeper for auxin-dependent lateral root initiation in Arabidopsis thaliana
BioProject PRJNA818879

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE199202_RAW.tar 4.7 Mb (http)(custom) TAR (of TAB)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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