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Series GSE199637 Query DataSets for GSE199637
Status Public on Oct 02, 2023
Title CYR61 orchestrates NASH fibrosis through SYK-NFκB-PDGF signaling in monocyte-derived macrophages [bulk RNA-seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Obesity is increasing worldwide and leads to a multitude of metabolic diseases including non-alcoholic fatty liver disease (NAFLD) and non-alcoholic steatosis (NASH). Here we examine the role of CYR61 in liver fibrosis and inflammation and its potential as a therapeutic target. Loss of CYR61 during NASH injury improves glucose tolerance, decreases liver inflammation and reduces fibrosis. CYR61 activates signaling in monocytes and monocyte-derived macrophages promoting a pro-inflammatory/pro-fibrotic phenotype through a CYR61/SYK/NFKB signaling cascade. In vitro, CYR61 activates Pdgfa and Pdgfb expression in macrophages in a NFκB-dependent manner. Ultimately, we identify a potential therapeutic for NASH: a CYR61-blocking antibody that reduces fibrotic injury and CYR61-driven signaling in macrophages in vitro and in vivo. This study demonstrates that CYR61 is a key driver of liver inflammation and fibrosis and a strong therapeutic target for treatment of NAFLD/NASH.
 
Overall design mRNA profiles of two conditions: Control and Cyr61 KO, with three repeats each.
 
Contributor(s) Mooring M, Luukkonen P, Liu S, Balogun O, Mo R, Nejak-Bowen K, Poyurovsky M, Booth C, Konnikova L, Shulman GI, Yimlamai D
Citation(s) 37756381
Submission date Mar 28, 2022
Last update date Oct 03, 2023
Contact name Shuchang Liu
E-mail(s) shl96@pitt.edu
Organization name University of Pittsburgh
Department Pathology
Street address 203 Lothrop Street
City Pittsburgh
State/province PA
ZIP/Postal code 15261
Country USA
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (6)
GSM5979696 Bulk RNAseq CTRL rep1
GSM5979697 Bulk RNAseq CTRL rep2
GSM5979698 Bulk RNAseq CTRL rep3
This SubSeries is part of SuperSeries:
GSE199640 CYR61 orchestrates NASH fibrosis through SYK-NFκB-PDGF signaling in monocyte-derived macrophages
Relations
BioProject PRJNA820908

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Supplementary file Size Download File type/resource
GSE199637_RAW.tar 1020.0 Kb (http)(custom) TAR (of TAB)
GSE199637_STAR_count.csv.gz 285.0 Kb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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