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Series GSE199638 Query DataSets for GSE199638
Status Public on Oct 02, 2023
Title CYR61 orchestrates NASH fibrosis through SYK-NFκB-PDGF signaling in monocyte-derived macrophages [10X single-cell RNA-seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Obesity is increasing worldwide and leads to a multitude of metabolic diseases including non-alcoholic fatty liver disease (NAFLD) and non-alcoholic steatosis (NASH). Here we examine the role of CYR61 in liver fibrosis and inflammation and its potential as a therapeutic target. Loss of CYR61 during NASH injury improves glucose tolerance, decreases liver inflammation and reduces fibrosis. CYR61 activates signaling in monocytes and monocyte-derived macrophages promoting a pro-inflammatory/pro-fibrotic phenotype through a CYR61/SYK/NFKB signaling cascade. In vitro, CYR61 activates Pdgfa and Pdgfb expression in macrophages in a NFκB-dependent manner. Ultimately, we identify a potential therapeutic for NASH: a CYR61-blocking antibody that reduces fibrotic injury and CYR61-driven signaling in macrophages in vitro and in vivo. This study demonstrates that CYR61 is a key driver of liver inflammation and fibrosis and a strong therapeutic target for treatment of NAFLD/NASH.
Overall design Single-cell RNA-seq by 10X platform of two conditions: Control and KO, with two repeats each.
Contributor(s) Mooring M, Luukkonen P, Liu S, Balogun O, Mo R, Nejak-Bowen K, Poyurovsky M, Booth C, Konnikova L, Shulman GI, Yimlamai D
Citation(s) 37756381
Submission date Mar 28, 2022
Last update date Oct 03, 2023
Contact name Shuchang Liu
Organization name University of Pittsburgh
Department Pathology
Street address 203 Lothrop Street
City Pittsburgh
State/province PA
ZIP/Postal code 15261
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (4)
GSM5979702 10X scRNA-seq CTRL rep1
GSM5979703 10X scRNA-seq CTRL rep2
GSM5979704 10X scRNA-seq KO rep1
This SubSeries is part of SuperSeries:
GSE199640 CYR61 orchestrates NASH fibrosis through SYK-NFκB-PDGF signaling in monocyte-derived macrophages
BioProject PRJNA820907

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE199638_RAW.tar 88.9 Mb (http)(custom) TAR (of MTX, TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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