NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE199638 Query DataSets for GSE199638
Status Public on Oct 02, 2023
Title CYR61 orchestrates NASH fibrosis through SYK-NFκB-PDGF signaling in monocyte-derived macrophages [10X single-cell RNA-seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Obesity is increasing worldwide and leads to a multitude of metabolic diseases including non-alcoholic fatty liver disease (NAFLD) and non-alcoholic steatosis (NASH). Here we examine the role of CYR61 in liver fibrosis and inflammation and its potential as a therapeutic target. Loss of CYR61 during NASH injury improves glucose tolerance, decreases liver inflammation and reduces fibrosis. CYR61 activates signaling in monocytes and monocyte-derived macrophages promoting a pro-inflammatory/pro-fibrotic phenotype through a CYR61/SYK/NFKB signaling cascade. In vitro, CYR61 activates Pdgfa and Pdgfb expression in macrophages in a NFκB-dependent manner. Ultimately, we identify a potential therapeutic for NASH: a CYR61-blocking antibody that reduces fibrotic injury and CYR61-driven signaling in macrophages in vitro and in vivo. This study demonstrates that CYR61 is a key driver of liver inflammation and fibrosis and a strong therapeutic target for treatment of NAFLD/NASH.
 
Overall design Single-cell RNA-seq by 10X platform of two conditions: Control and KO, with two repeats each.
 
Contributor(s) Mooring M, Luukkonen P, Liu S, Balogun O, Mo R, Nejak-Bowen K, Poyurovsky M, Booth C, Konnikova L, Shulman GI, Yimlamai D
Citation(s) 37756381
Submission date Mar 28, 2022
Last update date Oct 03, 2023
Contact name Shuchang Liu
E-mail(s) shl96@pitt.edu
Organization name University of Pittsburgh
Department Pathology
Street address 203 Lothrop Street
City Pittsburgh
State/province PA
ZIP/Postal code 15261
Country USA
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (4)
GSM5979702 10X scRNA-seq CTRL rep1
GSM5979703 10X scRNA-seq CTRL rep2
GSM5979704 10X scRNA-seq KO rep1
This SubSeries is part of SuperSeries:
GSE199640 CYR61 orchestrates NASH fibrosis through SYK-NFκB-PDGF signaling in monocyte-derived macrophages
Relations
BioProject PRJNA820907

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE199638_RAW.tar 88.9 Mb (http)(custom) TAR (of MTX, TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap