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Series GSE200157 Query DataSets for GSE200157
Status Public on Apr 30, 2023
Title Deconstructing replicative senescence heterogeneity of human mesenchymal stem cells at single cell resolution reveals therapeutically targetable senescent cell sub-populations
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Cellular senescence is characterised by a state of permanent cell cycle arrest. While cellular senescence is a recognised driver of organismal ageing, the extent of heterogeneity within and between different senescent cell populations remains largely unclear. Here we combined cell biological and single cell RNA-sequencing approaches to investigate heterogeneity of replicative senescence in human ESC-derived mesenchymal stem cells (esMSCs) as MSCs are the cell type of choice for the majority of current stem cell herapies and senescence of MSC is a recognized driver of organismal ageing. We identified three senescing esMSC subpopulations and their associated maker genes, allowing us to identify potential drug targets that may delay the emergence of senescent MSCs in vitro and perhaps in vivo in the future.
Overall design Mesenchymal stem cells undergoing replicative senescence. Three timepoints and three biological replicates in each timepoint. Over all 9 samples.
Contributor(s) Taherian Fard A, Mar JC, Wolvetang E
Citation(s) 37314668
Submission date Apr 04, 2022
Last update date Feb 14, 2024
Contact name Atefeh Taherian Fard
Organization name The University of Queensland
Street address St Lucia
City Brisbane
ZIP/Postal code 4072
Country Australia
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (45)
GSM6016109 H2HJKBGXC_Gen22_LG_D22
GSM6016110 H2HJKBGXC_Gen22_LG_D49
GSM6016111 H2HJKBGXC_Gen22_LG_D86
BioProject PRJNA823365

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GSE200157_GEO_TaherianFard_etal_MSC_senescence_processed.tar.gz 1.4 Gb (ftp)(http) TAR
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