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Series GSE20024 Query DataSets for GSE20024
Status Public on Aug 01, 2011
Title Mycobacteria-induced anaemia: a molecular approach reveals the involvement of NRAMP1 and lipocalin-2 but not hepcidin
Organism Mus musculus
Experiment type Expression profiling by array
Summary Anaemia is a frequent complication of chronic infectious diseases but the exact mechanisms by which it develops remain to be clarified. In the present work, we used a mouse model of mycobacterial infection to study molecular alterations of iron metabolism.
We show that four weeks after infection with Mycobacterium avium BALB/c mice exhibit a moderate anaemia, which cannot be explained by elevated hepatic hepcidin mRNA expression. Instead, the mice respond with increased mRNA expression of ferroportin (Slc40a1), ceruloplasmin (Cp), hemopexin (Hpx), heme-oxygenase-1 (Hmox1) and lipocalin-2 (Lcn2). Both the anemia and the mRNA expression changes of iron-related genes are largely absent in C.D2 mice which bear a functional allele of the Nramp1 gene. These data suggest that anaemia due to a chronic infection with M. avium develops independently of elevated hepcidin expression and possibly involves ferroportin and/or lipocalin-2.
 
Overall design A pool of five mice total RNAs for each condition was used in the experiments. Each two channel chip experiment was done in duplicates with swapped dyes.
 
Contributor(s) Rodrigues PN, Gomes SS, Neves JV, Gomes-Pereira S, Correia-Neves M, Nunes-Alves C, Stolte J, Sanchez M, Appelberg R, Muckenthaler MU, Gomes MS
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Submission date Jan 25, 2010
Last update date Mar 22, 2012
Contact name Maria Salome Gomes
E-mail(s) sgomes@ibmc.up.pt
Organization name Universidad do Porto
Department IBMC/ICBAS
Street address Rua do Campo Alegre 823
City Porto
ZIP/Postal code 4150-180
Country Portugal
 
Platforms (1)
GPL9971 Murine 0.6K,cDNA array Version 8.0
Samples (8)
GSM500910 BALB/c vs. BALB/c infected
GSM500911 BALB/c infected vs. BALB/c
GSM500912 C.D2 vs. C.D2 infected
Relations
BioProject PRJNA124105

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE20024_RAW.tar 7.6 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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