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Series GSE200483 Query DataSets for GSE200483
Status Public on Nov 01, 2022
Title RNA-seq on ES cells where DDX6 was knocked down by the AID method
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To degrade DDX6 acutely, we adopted an advanced protein degradation method, the auxin-induced degron (AID) system. For this purpose, we prepared an AID-tagged DDX6 and E3 ligase subunit of OsTIR1 in an engineered ES (D6AdP cell line). These deposited data are RNA-seq data on these D6AdP ES cells treated with the auxin analog (IAA) treatment for 0, 3, and 6 hours.
 
Overall design Single-end RNA-seq of polyA RNAs originally from 500-1000 ng of total RNAs extracted from the D6AdP ES cells treated with the auxin analog (IAA) treatment for 0, 3, and 6 hours, processed with the Truseq Stranded mRNA Library Prep kit (Illumina).
 
Contributor(s) Saga Y, Muraoka M
Citation(s) 36353942
Submission date Apr 08, 2022
Last update date Feb 02, 2023
Contact name Yumiko Saga
E-mail(s) ysaga@nig.ac.jp
Organization name National Institute of Genetics
Department Department of Gene Function and Phenomics
Lab Mammalian Development
Street address 1111 Yata
City Mishima
State/province Shizuoka
ZIP/Postal code 411-8540
Country Japan
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (6)
GSM6034885 DDX6-AID 0h-1
GSM6034886 DDX6-AID 3h-1
GSM6034887 DDX6-AID 6h-1
Relations
BioProject PRJNA824800

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE200483_Raw_gene_counts_matrix_FeatureCounts.txt.gz 436.7 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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