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| Status |
Public on Apr 18, 2022 |
| Title |
Temporal perturbation of histone deacetylase activity reveals a requirement for HDACs 1-3 in mes-endoderm cell differentiation |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We investigate the genome-wide chromatin landscape during mes-endoderm and cardiac progenitor specification to reveal dynamic changes to chromatin accessibility upon HDAC1-3 inhibition.
To evaluate the changes to chromatin accessibility upon inhibition of HDACs 1-3 during cardiac lineage specification, we performed ATAC-seq using the Omni-ATAC protocol (Corces et al., 2017) on Day 5 differentiated hiPSCs treated for 48 hours using small molecule HDAC inhibitors with isozyme selectivity
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| Overall design |
ATAC-seq analysis of cells at Day 5 of cardiac-directed hiPSC differentiation treated for 48 hours with HDAC inhibitors (1 µM MS275 and 1 µM BRD4884) (3 biological replicates per condition)
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| Contributor(s) |
Sinniah E, Naval-Sabchez M, Chen X, Nefzger C, Palpant NJ |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Apr 14, 2022 |
| Last update date |
Apr 22, 2022 |
| Contact name |
Nathan J. Palpant |
| Organization name |
University of Queensland
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| Street address |
306 Carmody Rd
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| City |
Brisbane |
| State/province |
QLD |
| ZIP/Postal code |
4067 |
| Country |
Australia |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (9)
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| GSM6043947 |
Replicate 3; BRD4884 treated; Day 5 differentiated hiPSCs |
| GSM6043948 |
Replicate 2; BRD4884 treated Day 5 differentiated hiPSCs |
| GSM6043949 |
Replicate 1; BRD4884 treated Day 5 differentiated hiPSCs |
| GSM6043950 |
Replicate 3; Control Day 5 differentiated hiPSCs |
| GSM6043951 |
Replicate 2; Control Day 5 differentiated hiPSCs |
| GSM6043952 |
Replicate 1; Control Day 5 differentiated hiPSCs |
| GSM6043953 |
Replicate 3; MS275 treated Day 5 differentiated hiPSCs |
| GSM6043954 |
Replicate 2; MS275 treated Day 5 differentiated hiPSCs |
| GSM6043955 |
Replicate 1; MS275 treated Day 5 differentiated hiPSCs |
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| Relations |
| BioProject |
PRJNA826850 |
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