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Series GSE20125 Query DataSets for GSE20125
Status Public on Jul 19, 2010
Title Transcriptome analysis of human Wharton’s jelly stem cells: meta-analysis
Organism Homo sapiens
Experiment type Third-party reanalysis
Expression profiling by array
Summary Human Wharton’s jelly stem cells (hWJSCs) are derived ethically in large amounts from the umbilical cord matrix. Besides their differentiation capabilities, WJSCs also display a notable lack of ability to form teratoma. hWJSCs have been shown to exert immunomodulatory effects and have recently been reported to kill or diminish cancer cell growth. These characteristics are important considerations for their use in cell therapy. In this transcriptome analysis, hWJSCs were profiled using Affymetrix DNA microarrays and compared to a panel of human stem cells and stromal cells. Although hWJSCs are multipotent, they expressed very low levels of the majority of stem cell markers, including POU5F1, NANOG, SOX2 and LIN28. BIRC5 has recently been shown to be required for teratoma formation in SCID mice. The lower levels of BIRC5 expression in hWJSCs compared to hESCs and the very low levels of stem cell markers might account for hWJSCs inability to form teratomas. IL12A which is known to be associated with the induction of apoptosis, was amongst the several cytokines identified to be significantly upregulated in hWJSCs. The ability of hWJSCs to compliment the host immune responses was further highlighted with the GO Biological Process analysis showing high association with immune system, chemotaxis and cell death. The ability to modulate immune responses confers hWJSCs an additional advantage in stem cell therapy and potentially allows hWJSCs as a form of treatment for cancer and immune disorders. In summary, the transcriptome profile of hWJSCs has provided indications on the genetic basis for their biological characteristics in immunomodulatory response, anti-cancer effects, and the lack of teratoma formation.
Overall design This 'meta-analysis' dataset contains hWJSCs and hFC that were generated by our group, and selected samples available from the public GEO database (human mesenchymal stem cells, umbilical vein endothelial cells, normal stroma, tumour stroma, fibroblast).
Contributor(s) Fong C, Chak L, Biswas A, Tan J, Gauthaman K, Chan W, Bongso A
Citation(s) 20602182
Submission date Feb 01, 2010
Last update date Mar 25, 2019
Contact name Woon-Khiong Chan
Organization name National University of Singapore
Department Biological Sciences
Lab Molecular Genetics Laboratory
Street address S2-05-19, 14 Science Drive 4
City Singapore
ZIP/Postal code 117543
Country Singapore
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (18)
GSM503622 D551_replicate1
GSM503623 D551_replicate2
GSM503624 WJ1_passage3_replicate1
This SubSeries is part of SuperSeries:
GSE20126 Transcriptome analysis of human Wharton’s jelly stem cells
Reanalysis of GSM194076
Reanalysis of GSM194077
Reanalysis of GSM194078
Reanalysis of GSM194079
Reanalysis of GSM194094
Reanalysis of GSM194095
Reanalysis of GSM194096
Reanalysis of GSM194097
Reanalysis of GSM239713
Reanalysis of GSM239715
Reanalysis of GSM239722
Reanalysis of GSM241199
Reanalysis of GSM241201
Reanalysis of GSM241203
Reanalysis of GSM250019
Reanalysis of GSM250020
Reanalysis of GSM250021
Reanalysis of GSM322376
Reanalysis of GSM322377
Reanalysis of GSM322374
Reanalysis of GSM322375
Reanalysis of GSM380010
Reanalysis of GSM340079
Reanalysis of GSM340080
Reanalysis of GSM340081
Reanalysis of GSM340085
Reanalysis of GSM340086
Reanalysis of GSM340087
Reanalysis of GSM143726
Reanalysis of GSM143727
Reanalysis of GSM176298
Reanalysis of GSM176299
Reanalysis of GSM265056
Reanalysis of GSM265057
Reanalysis of GSM265058
Reanalysis of GSM265059
Reanalysis of GSM418126
Reanalysis of GSM418127
Reanalysis of GSM418128
Reanalysis of GSM227523
Reanalysis of GSM227524
Reanalysis of GSM227525
Reanalysis of GSM227526
Reanalysis of GSM227527
Reanalysis of GSM227528
Reanalysis of GSM227529
Reanalysis of GSM227530
Reanalysis of GSM227531
Reanalysis of GSM227532
Reanalysis of GSM227533
Reanalysis of GSM227534
Reanalysis of GSM227535
Reanalysis of GSM447155
Reanalysis of GSM447156
Reanalysis of GSM447157
Reanalysis of GSM447158
Reanalysis of GSM447159
Reanalysis of GSM447176
Reanalysis of GSM447178
Reanalysis of GSM447172
Reanalysis of GSM447173
Reanalysis of GSM447174
Reanalysis of GSM239766
Reanalysis of GSM239769
Reanalysis of GSM239801
Reanalysis of GSM248209
Reanalysis of GSM248210
BioProject PRJNA129779

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Supplementary file Size Download File type/resource
GSE20125_RAW.tar 72.9 Mb (http)(custom) TAR (of CEL)
GSE20125_meta-analysis.txt.gz 17.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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