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Series GSE20125

Query DataSets for GSE20125

Status

Public on Jul 19, 2010

Title

Transcriptome analysis of human Wharton’s jelly stem cells: meta-analysis

Organism

Homo sapiens

Experiment type

Third-party reanalysis
Expression profiling by array

Summary

Human Wharton’s jelly stem cells (hWJSCs) are derived ethically in large amounts from the umbilical cord matrix. Besides their differentiation capabilities, WJSCs also display a notable lack of ability to form teratoma. hWJSCs have been shown to exert immunomodulatory effects and have recently been reported to kill or diminish cancer cell growth. These characteristics are important considerations for their use in cell therapy. In this transcriptome analysis, hWJSCs were profiled using Affymetrix DNA microarrays and compared to a panel of human stem cells and stromal cells. Although hWJSCs are multipotent, they expressed very low levels of the majority of stem cell markers, including POU5F1, NANOG, SOX2 and LIN28. BIRC5 has recently been shown to be required for teratoma formation in SCID mice. The lower levels of BIRC5 expression in hWJSCs compared to hESCs and the very low levels of stem cell markers might account for hWJSCs inability to form teratomas. IL12A which is known to be associated with the induction of apoptosis, was amongst the several cytokines identified to be significantly upregulated in hWJSCs. The ability of hWJSCs to compliment the host immune responses was further highlighted with the GO Biological Process analysis showing high association with immune system, chemotaxis and cell death. The ability to modulate immune responses confers hWJSCs an additional advantage in stem cell therapy and potentially allows hWJSCs as a form of treatment for cancer and immune disorders. In summary, the transcriptome profile of hWJSCs has provided indications on the genetic basis for their biological characteristics in immunomodulatory response, anti-cancer effects, and the lack of teratoma formation.

Overall design

This 'meta-analysis' dataset contains hWJSCs and hFC that were generated by our group, and selected samples available from the public GEO database (human mesenchymal stem cells, umbilical vein endothelial cells, normal stroma, tumour stroma, fibroblast).

Contributor(s)

Fong C, Chak L, Biswas A, Tan J, Gauthaman K, Chan W, Bongso A

Citation(s)

20602182

Submission date

Feb 01, 2010

Last update date

Mar 25, 2019

Contact name

Woon-Khiong Chan

E-mail(s)

dbscwk@nus.edu.sg, g0500139@nus.edu.sg

Organization name

National University of Singapore

Department

Biological Sciences

Lab

Molecular Genetics Laboratory

Street address

S2-05-19, 14 Science Drive 4

City

Singapore

ZIP/Postal code

117543

Country

Singapore

Platforms (1)

GPL570

[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array

Samples (18)

More...

GSM503622	D551_replicate1
GSM503623	D551_replicate2
GSM503624	WJ1_passage3_replicate1

This SubSeries is part of SuperSeries:

GSE20126

Transcriptome analysis of human Wharton’s jelly stem cells

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BioProject

Download family	Format
SOFT formatted family file(s)	SOFT
MINiML formatted family file(s)	MINiML
Series Matrix File(s)	TXT

Supplementary file	Size	Download	File type/resource
GSE20125_RAW.tar	72.9 Mb	(http)(custom)	TAR (of CEL)
GSE20125_meta-analysis.txt.gz	17.4 Mb	(ftp)(http)	TXT
Processed data included within Sample table