|
| Status |
Public on Mar 01, 2014 |
| Title |
aCGH profiles of parental and oxaliplatin resistant colorectal cancer and ovarian cancer cell lines |
| Organism |
Homo sapiens |
| Experiment type |
Genome variation profiling by array
|
| Summary |
Oxaliplatin resistance was induced in 2 colorectal cancer cell lines (LoVo-92, wt-p53 and LoVo-Li, functionally inactive p53) and one ovarian cancer cell line (A2780, wt-p53). Resistance was induced by weekly exposure to oxaliplatin for 4 hrs or 72 hrs with increasing concentrations for a period of 7 months
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| Overall design |
Genomic DNA of oxaliplatin and cisplatin resistant colorectal cancer and ovarian cancer cell lines as well as the parental cell lines were labeled and subsequently hybridized against pooled reference DNA of healthy volunteers of the opposite gender using across array hybridization. Extracted raw-data were normalised and smoothend using the R-script NOWAVE resulting in normalised log2 ratio profiles of resistant cell line versus parental cell line and parental cell line versus reference DNA.
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| Contributor(s) |
Noordhuis P, Laan AC, Losekoot N, van de Born K, Eijk P, IJlstra B, Peters GJ |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Feb 02, 2010 |
| Last update date |
Mar 01, 2014 |
| Contact name |
Daoud Sie |
| E-mail(s) |
d.sie@vumc.nl
|
| Phone |
+31 20 4442428
|
| Organization name |
Vrije Universiteit Medical Center
|
| Department |
Pathology
|
| Lab |
Microarray Core Facility
|
| Street address |
De Boelelaan 1117
|
| City |
Amsterdam |
| ZIP/Postal code |
1081 HV |
| Country |
Netherlands |
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| Platforms (1) |
| GPL5477 |
Agilent-014950 Human Genome CGH Microarray 4x44K (Feature Number version) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA125563 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE20144_MAL_251495011798_S01_CGH-v4_95_Feb07_1_1.txt.gz |
14.2 Mb |
(ftp)(http) |
TXT |
| GSE20144_MAL_251495011798_S01_CGH-v4_95_Feb07_1_4.txt.gz |
14.1 Mb |
(ftp)(http) |
TXT |
| GSE20144_MAL_251495013768_S01_CGH-v4_95_Feb07_1_1.txt.gz |
13.4 Mb |
(ftp)(http) |
TXT |
| GSE20144_MAL_251495013768_S01_CGH-v4_95_Feb07_1_2.txt.gz |
13.3 Mb |
(ftp)(http) |
TXT |
| GSE20144_MAL_251495014120_S01_CGH-v4_95_Feb07_1_1.txt.gz |
13.1 Mb |
(ftp)(http) |
TXT |
| GSE20144_MAL_251495014120_S01_CGH-v4_95_Feb07_1_2.txt.gz |
13.1 Mb |
(ftp)(http) |
TXT |
| GSE20144_MAL_251495014120_S01_CGH-v4_95_Feb07_1_3.txt.gz |
13.0 Mb |
(ftp)(http) |
TXT |
| GSE20144_MAL_251495014120_S01_CGH-v4_95_Feb07_1_4.txt.gz |
13.0 Mb |
(ftp)(http) |
TXT |
| GSE20144_Raw_File_Dye_Assignments.txt.gz |
227 b |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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