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Series GSE20144 Query DataSets for GSE20144
Status Public on Mar 01, 2014
Title aCGH profiles of parental and oxaliplatin resistant colorectal cancer and ovarian cancer cell lines
Organism Homo sapiens
Experiment type Genome variation profiling by array
Summary Oxaliplatin resistance was induced in 2 colorectal cancer cell lines (LoVo-92, wt-p53 and LoVo-Li, functionally inactive p53) and one ovarian cancer cell line (A2780, wt-p53). Resistance was induced by weekly exposure to oxaliplatin for 4 hrs or 72 hrs with increasing concentrations for a period of 7 months
Overall design Genomic DNA of oxaliplatin and cisplatin resistant colorectal cancer and ovarian cancer cell lines as well as the parental cell lines were labeled and subsequently hybridized against pooled reference DNA of healthy volunteers of the opposite gender using across array hybridization. Extracted raw-data were normalised and smoothend using the R-script NOWAVE resulting in normalised log2 ratio profiles of resistant cell line versus parental cell line and parental cell line versus reference DNA.
Contributor(s) Noordhuis P, Laan AC, Losekoot N, van de Born K, Eijk P, IJlstra B, Peters GJ
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Submission date Feb 02, 2010
Last update date Mar 01, 2014
Contact name Daoud Sie
Phone +31 20 4442428
Organization name Vrije Universiteit Medical Center
Department Pathology
Lab Microarray Core Facility
Street address De Boelelaan 1117
City Amsterdam
ZIP/Postal code 1081 HV
Country Netherlands
Platforms (1)
GPL5477 Agilent-014950 Human Genome CGH Microarray 4x44K (Feature Number version)
Samples (10)
GSM503023 LoVo-92/4OHP oxaliplatin resistant
GSM503030 LoVo-92/cOHP oxaliplatin resistant
GSM503032 Lovo-Li/4OHP oxaliplatin resistant
BioProject PRJNA125563

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE20144_MAL_251495011798_S01_CGH-v4_95_Feb07_1_1.txt.gz 14.2 Mb (ftp)(http) TXT
GSE20144_MAL_251495011798_S01_CGH-v4_95_Feb07_1_4.txt.gz 14.1 Mb (ftp)(http) TXT
GSE20144_MAL_251495013768_S01_CGH-v4_95_Feb07_1_1.txt.gz 13.4 Mb (ftp)(http) TXT
GSE20144_MAL_251495013768_S01_CGH-v4_95_Feb07_1_2.txt.gz 13.3 Mb (ftp)(http) TXT
GSE20144_MAL_251495014120_S01_CGH-v4_95_Feb07_1_1.txt.gz 13.1 Mb (ftp)(http) TXT
GSE20144_MAL_251495014120_S01_CGH-v4_95_Feb07_1_2.txt.gz 13.1 Mb (ftp)(http) TXT
GSE20144_MAL_251495014120_S01_CGH-v4_95_Feb07_1_3.txt.gz 13.0 Mb (ftp)(http) TXT
GSE20144_MAL_251495014120_S01_CGH-v4_95_Feb07_1_4.txt.gz 13.0 Mb (ftp)(http) TXT
GSE20144_Raw_File_Dye_Assignments.txt.gz 227 b (ftp)(http) TXT
Processed data included within Sample table

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