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Series GSE201936 Query DataSets for GSE201936
Status Public on Dec 01, 2023
Title Utilizing Omomyc to Target MYCN-Amplified Diffuse Intrinsic Pontine Gliomas
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Genomic studies have shown that several types of pediatric brain tumors have dysregulation of MYC family members (c-MYC and MYCN). In fact, it has been estimated that about 50% of all human cancers have increased MYC, and this is correlated with tumor aggression and poor survival. Here we focus on targeting the MYCN oncogene in diffuse intrinsic pontine glioma (DIPG). DIPG has the worst prognosis of any pediatric brain tumor and is in desperate need of viable treatment options. Here we demonstrate that using a 90-amino acid peptide, termed “Omomyc” we are able to down-regulate endogenous MYCN and inhibit growth of MYCN overexpressing DIPG cells. Furthermore, Omomyc binds to promoter regions normally occupied by MYCN to affect transcription of a subset of genes including PLK1 and LDHA, among others. In vivo expression of Omomyc in DIPG cells implanted in mice slows disease progression, suggesting that MYCN targets are important for driving rapid tumor growth and may offer insight into potential targets for treatment of MYCN driven DIPGs.
 
Overall design RNASeq was performed on four different samples. Each sample was prepared as a singleton. Three samples are controls/references (1-SM-vumc-GFP_S1, 2-SM-vumc-GFP-dox_S2, 3-SM-vumc-omo_S3) and one sample is treated/test (4-SM-vumc-omo-dox_S4).
 
Contributor(s) Morris SM, Sarthy J, Arora S, Freie B, Carroll P
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Submission date Apr 30, 2022
Last update date Dec 01, 2023
Contact name Sonali Arora
E-mail(s) sarora@fredhutch.org
Organization name FHCRC
Street address 1100 Fairview Ave N,
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (4)
GSM6084321 1-SM-vumc-GFP_S1
GSM6084322 2-SM-vumc-GFP-dox_S2
GSM6084323 3-SM-vumc-omo_S3
Relations
BioProject PRJNA833664

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE201936_RAW.tar 1020.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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