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| Status |
Public on Mar 04, 2024 |
| Title |
Gene expression profile of alveolar macrophages (AM) from bleomycin treated mice. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Osteopontin (OPN, Spp1-/-) is considered detrimental in pulmonary fibrosis. However, effect of OPN produced by AMs during pulmonary fibrosis is not known. We used bulk RNA sequencing to analyze the effect of OPN in AMs from bleomycin treated mice.
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| Overall design |
Alveolar macrophages of wild type (WT) and osteopontin knock-out (Spp1-/-) mice were isolated by Fluorescence-activated cell sorting (FACS) according to the cell surface markers and analyzed using scRNAseq.
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| Contributor(s) |
Nupur A |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
May 05, 2022 |
| Last update date |
Mar 04, 2024 |
| Contact name |
Mari Shinohara |
| E-mail(s) |
nupuraggarwal88@gmail.com
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| Organization name |
Duke University Medical School
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| Department |
Immunology
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| Lab |
Shinohara
|
| Street address |
207 research drive
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| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27703 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA835498 |
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