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Series GSE20241 Query DataSets for GSE20241
Status Public on Mar 01, 2011
Title Genome-wide analysis of Dnmt3L heterozygous and wildtype spermatocytes and spermatids
Organism Mus musculus
Experiment type Expression profiling by array
Summary Comparison of gene expression differences between Dnmt3L heterozygous and wildtype pachytene spermatocytes, and similarly between Dnmt3L heterozygous and wildtype round spermatids which were isolated from the Dnmt3L knockout mouse line. This array was conducted to address the hypothesis that Dnmt3L heterozygosity results in deregulated gene expression within spermatocytes and spermatids. Results show that Dnmt3L heterozygosity causes numerous genes to be differentially regulated on a genome-wide level, showing that DNMT3L has an important role in regulating gene expression within these male germ cells.
 
Overall design Three preparations each of Dnmt3L purified wildtype spermatocytes, wildtype spermatids, heterozygous spermatids, and two preparations of heterozygous spermatocytes were isolated for a total of 11 samples. Each preparation was made up of cells isolated from 10 mice.
 
Contributor(s) Zamudio NM, O'Bryan MK, Smyth GK, Law C
Citation(s) 21483837
Submission date Feb 09, 2010
Last update date Mar 22, 2012
Contact name Gordon K Smyth
E-mail(s) smyth@wehi.edu.au
Phone (+61 3) 9345 2326
Fax (+61 3) 9347 0852
URL http://www.wehi.edu.au
Organization name Walter and Eliza Hall Institute of Medical Research
Department Bioinformatics
Lab Smyth
Street address 1G Royal Pde
City Parkville
State/province Vic
ZIP/Postal code 3052
Country Australia
 
Platforms (1)
GPL6481 Illumina mouse-6 v1.1 expression beadchip [Array_Address_Id version]
Samples (11)
GSM507261 Dnmt3L wildtype spermatocytes 1
GSM507262 Dnmt3L wildtype spermatocytes 2
GSM507263 Dnmt3L wildtype spermatocytes 3
Relations
BioProject PRJNA125745

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE20241_matrix_RAW.txt.gz 2.3 Mb (ftp)(http) TXT
Processed data included within Sample table

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