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Series GSE202485 Query DataSets for GSE202485
Status Public on Aug 01, 2022
Title P450 oxidoreductase (POR) Regulates Barrier Maturation by Mediating Retinoic Acid Metabolism in a Model of the Human BBB
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The blood-brain barrier (BBB) is a multicellular neurovascular unit (NVU) that allows the selective passage of necessary molecules into the central nervous system (CNS), while limiting the entry of neurotoxins and most drugs. A crosstalk with pericytes and neural cells mediates the acquisition of specialized properties, such as the formation of tight junctions (TJs), in brain microvascular endothelial cells (BMECs). TJs limit the paracellular passage of solutes, thereby regulating CNS homeostasis. However, the mechanisms by which NVU cells communicate to mediate TJ formation remains a mystery. Retinoic acid (RA), a key signaling molecule during vertebrate embryonic development, is commonly used to enhance functional barrier properties in in vitro BBB models. However, its physiological relevance and affected pathways are not fully understood.
 
Overall design induced pluripotent stem (iPS)-derived brain microvascular endothelial-like cells, from healthy controls, POR-deficient patients and POR mutated lines . Each line was differentiated with 0, 0.25, 1, 10 uM of all-trans retinoic acd (RA), in triplicates . These triplicates include 2 technical duplicates and an one additional biological replicate.
 
Contributor(s) Vatine GD
Citation(s) 35961311
Submission date May 08, 2022
Last update date Oct 04, 2022
Contact name Inbar Plaschkes
Organization name HUJI
Department The Faculty of Medicine - Ein Kerem
Street address The Hebrew University of Jerusalem - Ein Kerem
City Jerusalem
ZIP/Postal code 91120
Country Israel
 
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (36)
GSM6123053 Control iPS-derived BMECs differentuated with 0 uM Retinoic acid [C0_a]
GSM6123054 Control iPS-derived BMECs differentuated with 0 uM Retinoic acid [C0_b]
GSM6123055 Control iPS-derived BMECs differentuated with 0 uM Retinoic acid [C0_c]
Relations
BioProject PRJNA836276

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Supplementary file Size Download File type/resource
GSE202485_DESeq2_normc_all_dup_rem.txt.gz 2.9 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record
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