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Series GSE20249 Query DataSets for GSE20249
Status Public on Apr 13, 2011
Title Genome-wide profiling of RNA associated to the MSL-complex in Drosophila melanogaster
Organism Drosophila melanogaster
Experiment type Expression profiling by genome tiling array
Summary In Drosophila, the global increase in transcription from the X chromosome in males to compensate for its monosomy is mediated by the male-specific-lethal complex (MSL-C) consisting of five proteins and two non-coding RNAs, roX1 and roX2. After an initial sequence dependent recognition by the MSL-C of 150-300 high affinity sites, the spreading to the majority of the X-linked genes depends on local MSL-C concentration and active transcription. Here we ask whether any additional RNA species are associated to the MSL-C. No additional roX were found but a strong association was found between the msl2 mRNA and the MSL-C. Based on our results we propose a model in which a non-chromatin associated partial or complete MSL-C titrates newly transcribed msl2 mRNA and thus feed-back regulates the amount of available MSL-C.
 
Overall design In total 12 samples; 4 Input files (4 different conditions) with the corresponding 8 RIP samples (2 different antibodies, same 4 conditions as Input)
 
Contributor(s) Johansson A, Allgardsson A, Stenberg P, Larsson J
Citation(s) 21551218, 22473994
Submission date Feb 09, 2010
Last update date Jul 08, 2015
Contact name Jan Larsson
E-mail(s) jan.larsson@molbiol.umu.se
Organization name Umea University
Department Molecular Biology
Lab Jan Larsson
Street address Umea University
City Umea
ZIP/Postal code SE-90187
Country Sweden
 
Platforms (1)
GPL6629 [DM_tiling2_MR] Affymetrix Drosophila Tiling 2.0R Array
Samples (12)
GSM507467 MSL2_RIP_N1
GSM507468 MSL2_RIP_N3
GSM507469 MSL2_RIP_N6
This SubSeries is part of SuperSeries:
GSE28519 Pof mutant
Relations
BioProject PRJNA141697

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE20249_RAW.tar 568.0 Mb (http)(custom) TAR (of CEL, CHP, TXT)
Processed data provided as supplementary file

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