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Series GSE20249 Query DataSets for GSE20249
Status Public on Apr 13, 2011
Title Genome-wide profiling of RNA associated to the MSL-complex in Drosophila melanogaster
Organism Drosophila melanogaster
Experiment type Expression profiling by genome tiling array
Summary In Drosophila, the global increase in transcription from the X chromosome in males to compensate for its monosomy is mediated by the male-specific-lethal complex (MSL-C) consisting of five proteins and two non-coding RNAs, roX1 and roX2. After an initial sequence dependent recognition by the MSL-C of 150-300 high affinity sites, the spreading to the majority of the X-linked genes depends on local MSL-C concentration and active transcription. Here we ask whether any additional RNA species are associated to the MSL-C. No additional roX were found but a strong association was found between the msl2 mRNA and the MSL-C. Based on our results we propose a model in which a non-chromatin associated partial or complete MSL-C titrates newly transcribed msl2 mRNA and thus feed-back regulates the amount of available MSL-C.
Overall design In total 12 samples; 4 Input files (4 different conditions) with the corresponding 8 RIP samples (2 different antibodies, same 4 conditions as Input)
Contributor(s) Johansson A, Allgardsson A, Stenberg P, Larsson J
Citation(s) 21551218, 22473994
Submission date Feb 09, 2010
Last update date Jul 08, 2015
Contact name Jan Larsson
Organization name Umea University
Department Molecular Biology
Lab Jan Larsson
Street address Umea University
City Umea
ZIP/Postal code SE-90187
Country Sweden
Platforms (1)
GPL6629 [DM_tiling2_MR] Affymetrix Drosophila Tiling 2.0R Array
Samples (12)
GSM507467 MSL2_RIP_N1
GSM507468 MSL2_RIP_N3
GSM507469 MSL2_RIP_N6
This SubSeries is part of SuperSeries:
GSE28519 Pof mutant
BioProject PRJNA141697

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE20249_RAW.tar 568.0 Mb (http)(custom) TAR (of CEL, CHP, TXT)
Processed data provided as supplementary file

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