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Series GSE202699 Query DataSets for GSE202699
Status Public on Aug 01, 2022
Title Generation of functional rat spermatozoa in sterile mice utilizing blastocyst complementation with pluripotent stem cells [scRNAseq_chimera]
Organisms Mus musculus; Rattus norvegicus
Experiment type Expression profiling by high throughput sequencing
Summary Pluripotent stem cells (PSCs) provide a powerful tool to produce transgenic animals for biomedical research. However, impaired PSC contribution to chimerism and most notably the germline oftentimes impedes production of genetically modified animals, rendering techniques that expediate PSC contribution to the germline highly desirable. Blastocyst complementation denotes a technique which purposes to generate organs, tissues or cells in animal chimeras via microinjection of PSCs into genetically compromised blastocyst-stage embryos. Here we report on successful blastocyst complementation of the male germline in adult chimeras following microinjection of mouse or rat PSCs into mouse blastocysts mutated for Tsc22d3, an essential gene for spermatozoa production. Microinjection of mouse embryonic stem cells (ESCs) or induced pluripotent stem cells (iPSCs) into Tsc22d3-KnockOut (KO) blastocyst-stage embryos gave rise to intraspecies chimeras embodying functional spermatozoa, which were solely derived from microinjected PSCs. Furthermore, microinjection of rat ESCs into Tsc22d3-KO mouse embryos gave rise to viable mouse-rat chimeras that exhibited extensive contribution of rat cells to various organs. Notably, multiple mouse-rat chimeras showed contribution of rat ESCs to the male germline, solely harboring rat spermatids and spermatozoa that were rat ESC-derived and could fertilize rat oocytes. Collectively, this study reports a method for exclusive production of functional germ cells of one species in another via blastocyst complementation with PSCs. Implications of this study extend to development of transgenic rats via sterile mice, and may further assist efforts to generate xenogeneic gametes from endangered animal species.
Overall design Samples were isolated from an adult mouse-rat chimera testis (host: goGermline, Ozgene. Donor: DAC8 rat ESCs) via the protocol published by Valli et al, Fertility and Sterility, 2014
Contributor(s) Zvick J, Ghosh A, Noé F, Bar-Nur O
Citation(s) 35931077
Submission date May 11, 2022
Last update date Oct 04, 2022
Contact name Ori Bar-Nur
Organization name ETH Zurich
Department Health Sciences and Technology
Lab Regenerative and Movement Biology
Street address Schorenstrasse 16
City Schwerzenbach
State/province Zurich
ZIP/Postal code 8603
Country Switzerland
Platforms (1)
GPL30020 Illumina NovaSeq 6000 (Mus musculus; Rattus norvegicus)
Samples (1)
GSM6131545 Mouse-rat chimera testis
This SubSeries is part of SuperSeries:
GSE167435 Generation of functional rat spermatozoa in sterile mice utilizing blastocyst complementation with pluripotent stem cells
BioProject PRJNA837067

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Supplementary file Size Download File type/resource
GSE202699_RAW.tar 132.1 Mb (http)(custom) TAR (of MTX, TSV)
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Raw data are available in SRA
Processed data provided as supplementary file

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