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Series GSE202757 Query DataSets for GSE202757
Status Public on Jun 08, 2023
Title Lactate-dependent Transcriptional Regulation Controls Mammalian Eye Morphogenesis [RNA-seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Mammalian retinal metabolism favors aerobic glycolysis. However, the role of glycolytic metabolism in retinal morphogenesis remains unknown. We report that aerobic glycolysis is necessary for the early stages of retinal development. Taking advantage of an unbiased approach that combines the use of eye organoids and single-cell RNA sequencing, we identify specific glucose transporters and glycolytic genes in retinal progenitors. Next, we determine that the optic vesicle territory of mouse embryos displays elevated levels of glycolytic activity. At the functional level, we show that removal of Glucose transporter 1 and Lactate dehydrogenase A gene activity from developing retinal progenitors arrests eye morphogenesis. Surprisingly, we uncover that lactate-mediated upregulation of key eye-field transcription factors is controlled by the epigenetic modification of histone H3 acetylation through histone deacetylase activity. Our results identify an unexpected bioenergetic independent role of lactate as a signaling molecule necessary for mammalian eye morphogenesis.
Overall design Comparative gene expression profiling analysis of RNA-seq data for the eye organoids and its treatments (control, GNE-140, and GNE-140 with lactate).
Contributor(s) Takata N, Embry R, Schipma M
Citation(s) 37452018
Submission date May 11, 2022
Last update date Sep 07, 2023
Contact name NOZOMU TAKATA
Organization name Northwestern University Feinberg School of Medicine
Department Center for Vascular and Developmental Biology
Street address 303 East Superior Street, 10-220
City Chicago
State/province Illinois
ZIP/Postal code 60611
Country USA
Platforms (1)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (12)
GSM6132407 Rax-GFP ESCs, control-1
GSM6132408 Rax-GFP ESCs, treated-1 (GNE-140)
GSM6132409 Rax-GFP ESCs, rescued-1 (GNE-140+lactate)
This SubSeries is part of SuperSeries:
GSE202759 Lactate-dependent Transcriptional Regulation Controls Mammalian Eye Morphogenesis.
BioProject PRJNA837221

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Supplementary file Size Download File type/resource
GSE202757_RAW.tar 2.3 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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