NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE202803 Query DataSets for GSE202803
Status Public on Aug 09, 2022
Title Duplicated ribosomal protein paralogs promote alternative translation and drug resistance
Organism Saccharomyces cerevisiae
Experiment type Expression profiling by high throughput sequencing
Summary Ribosomes are often seen as monolithic machines produced from uniformly regulated genes. However, in yeast most ribosomal proteins come from duplicated genes. Here, we demonstrate that gene duplication may serve as an adaption mechanism modulating the global proteome through the differential expression of ribosomal proteins paralogs after exposure to stress. Our data indicate that the yeast paralog pair of the ribosomal protein L7/uL30 produces two differentially acetylated proteins. Under normal conditions most ribosomes incorporate the hypo-acetylated major form favoring the translation of genes with short open reading frames. Exposure to drugs, on the other hand, increases the production of ribosomes carrying the hyper-acetylated minor paralog that increases translation of long reading frames. Many of these long genes encode cell wall proteins that increase drug resistance in a programed change in translation equilibrium. Together the data reveal a mechanism of translation control through the differential fates of near-identical ribosomal protein isoforms.
 
Overall design Total RNA and ribosome associated RNA from wild type cells and cells carrying a copy of either uL30A or uL30B on plasmid was sequenced.
 
Contributor(s) Malik-Ghulam M, Catala M, Reulet G, Scott MS, Abou Elela S
Citation(s) 35999447
Submission date May 11, 2022
Last update date Aug 31, 2022
Contact name Gerardo Ferbeyre
E-mail(s) g.ferbeyre@umontreal.ca
Organization name Université de Montréal
Department Biochimie et médecine moléculaire
Street address 2900 Boulevard Edouard-Montpetit
City Montréal
State/province QC
ZIP/Postal code H3T 1J4
Country Canada
 
Platforms (1)
GPL19756 Illumina NextSeq 500 (Saccharomyces cerevisiae)
Samples (18)
GSM6133446 WT_total_extract_N1
GSM6133447 WT_total_extract_N2
GSM6133448 WT_light_polysome_N1
Relations
BioProject PRJNA837278

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE202803_CPM.tsv.gz 609.1 Kb (ftp)(http) TSV
GSE202803_TPM.tsv.gz 489.7 Kb (ftp)(http) TSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap