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| Status |
Public on Oct 17, 2022 |
| Title |
3D Biocomposite Culture Enhances Differentiation of Dopamine-like Neurons from SH-SY5Y cells: A Model for Studying Parkinson’s Disease Phenotypes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Studies of underlying neurodegenerative processes in Parkinson’s Disease (PD) have traditionally utilized cell cultures grown on two-dimensional (2D) surfaces. Biomimetic three-dimensional (3D) cell culture platforms have been developed to better emulate features of the brain’s natural microenvironment. We here use our bioengineered brain-like tissue model, composed of a silk-hydrogel composite, to study the 3D microenvironment’s contributions on the development and performance of dopaminergic-like neurons (DLNs). Compared with 2D culture, SH-SY5Y cells differentiated in 3D microenvironments were enriched for DLNs concomitant with a reduction in proliferative capacity during the neurodevelopmental process. Additionally, the 3D DLN cultures were more sensitive to oxidative stresses elicited by the PD-related neurotoxin 1-methyl-4-phenylpyridinium (MPP). MPP induced transcriptomic profile changes specific to 3D-differentiated DLN cultures, replicating the dysfunction of neuronal signaling pathways and mitochondrial dynamics implicated in PD. Overall, this physiologically-relevant 3D platform resembles a useful tool for studying dopamine neuron biology and interrogating molecular mechanisms underlying neurodegeneration in PD.
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| Overall design |
Comparative gene expression profiling analysis of RNA-seq data for SH-SY5Y cells differentiated into dopamine-like neuron (DLN) cultures for 15 days using traditional 2D culture dishes and our bioengineered 3D silk-collagen platform. On day 13, 2D- and 3D-differentiated DLN cultures were treated with 1mM 1-methyl-4-phenylpyridinium (MPP) for 48 hours to investigate the effects of culture microenvironment on replicated features of PD.
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| Contributor(s) |
Fiore NJ, Tamer-Mahoney JD, Beheshti A, Nieland TJ, Kaplan DL |
| Citation |
Nicholas J. Fiore, Jackson D. Tamer-Mahoney, Afshin Beheshti, Thomas J.F. Nieland, David L. Kaplan, 3D biocomposite culture enhances differentiation of dopamine-like neurons from SH-SY5Y cells: A model for studying Parkinson's disease phenotypes, Biomaterials, Volume 290, 2022, 121858, ISSN 0142-9612, https://doi.org/10.1016/j.biomaterials.2022.121858
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| Submission date |
May 21, 2022 |
| Last update date |
Oct 20, 2022 |
| Contact name |
Nicholas James Fiore |
| E-mail(s) |
nicholasfiore963@gmail.com
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| Organization name |
Tufts University
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| Department |
Biomedical Engineering
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| Lab |
Kaplan Lab
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| Street address |
200 College Ave
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| City |
Medford |
| State/province |
MA |
| ZIP/Postal code |
02155 |
| Country |
USA |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (16)
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| GSM6175538 |
SH-SY5Y cells, CTRL, 2D, 4 |
| GSM6175539 |
SH-SY5Y cells, CTRL, 3D, 1 |
| GSM6175540 |
SH-SY5Y cells, CTRL, 3D, 2 |
| GSM6175541 |
SH-SY5Y cells, CTRL, 3D, 3 |
| GSM6175542 |
SH-SY5Y cells, CTRL, 3D, 4 |
| GSM6175543 |
SH-SY5Y cells, MPP, 2D, 1 |
| GSM6175544 |
SH-SY5Y cells, MPP, 2D, 2 |
| GSM6175545 |
SH-SY5Y cells, MPP, 2D, 3 |
| GSM6175546 |
SH-SY5Y cells, MPP, 2D, 4 |
| GSM6175547 |
SH-SY5Y cells, MPP, 3D, 1 |
| GSM6175548 |
SH-SY5Y cells, MPP, 3D, 2 |
| GSM6175549 |
SH-SY5Y cells, MPP, 3D, 3 |
| GSM6175550 |
SH-SY5Y cells, MPP, 3D, 4 |
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| Relations |
| BioProject |
PRJNA841191 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE203522_GEO_Normalized_Gene_Counts_DESeq2.csv.gz |
1.7 Mb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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