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| Status |
Public on May 18, 2010 |
| Title |
Increased leaf size: different means to an end (experiment 2) |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
The final size of plant organs such as leaves is tightly controlled by environmental and genetic factors that must spatially and temporally coordinate cell expansion and cell cycle activity. However this regulation of organ growth is still poorly understood. The aim of this study is to gain more insight in the genetic control of leaf size in Arabidopsis by performing a comparative analysis of transgenic lines that produce larger leaves under standardized environmental conditions. To this end, we selected five genes, belonging to different functional classes, that all positively affect leaf size when over-expressed: AVP1, GRF5, JAW, BRI1 and GA20OX1. We show that the increase in leaf area in these lines depends on leaf position and growth conditions and that all five lines affect leaf size differently. However, in all cases an increase in cell number is, entirely or predominantly, responsible for the leaf size enlargement. By means of analyses of hormone levels, transcriptome and metabolome we provide deeper insight in the molecular basis of the growth phenotype for the individual lines. A comparative analysis between them indicates that enhanced organ growth is governed by different, seemingly independent pathways. The analysis of transgenic lines simultaneously over-expressing two growth-enhancing genes further supports the concept that multiple pathways independently converge on organ size control in Arabidopsis.
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| Overall design |
Total RNAs were extracted using RNeasy plant mini kit (Qiagen) from vegetative part of seedlings at stage 1.03 grown in vitro. RNAs from three biological repeats of wild type (wt, control) and the GRF5 overexpressing line were submitted to ATH1 array hybridization.
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| Contributor(s) |
Gonzalez N, De Bodt S, Sulpice R, Jikumaru Y, Chae E, Van Daele T, De Milde L, Weigel D, Kamiya Y, Stitt M, Beemster GT, Inzé D |
| Citation(s) |
20460583 |
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| Submission date |
Feb 22, 2010 |
| Last update date |
Jan 18, 2013 |
| Contact name |
Nathalie Gonzalez |
| E-mail(s) |
nagon@psb.vib-ugent.be
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| Phone |
+32 (0)9 331 39 56
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| Organization name |
VIB
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| Department |
Plant System Biology
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| Lab |
System Biology of Yield
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| Street address |
Technologiepark 927
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| City |
Gent |
| ZIP/Postal code |
9052 |
| Country |
Belgium |
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| Platforms (1) |
| GPL8455 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array - custom CDF (tinesath1cdf) |
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| Samples (9)
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| GSM513096 |
wild type, biological rep1 (expt 2) |
| GSM513097 |
wild type, biological rep2 (expt 2) |
| GSM513098 |
wild type, biological rep3 (expt 2) |
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| This SubSeries is part of SuperSeries: |
| GSE20458 |
Increased leaf size: different means to an end |
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| Relations |
| BioProject |
PRJNA129871 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE20456_RAW.tar |
19.1 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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