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Series GSE20464 Query DataSets for GSE20464
Status Public on Feb 25, 2010
Title Identification of Aneuploidy-Tolerating Mutations
Organism Saccharomyces cerevisiae
Experiment type Expression profiling by array
Genome variation profiling by array
Summary Aneuploidy causes a proliferative disadvantage in all normal cells analyzed to date, yet this condition is associated with a disease characterized by unabated proliferative potential, cancer. The mechanisms that allow cancer cells to tolerate the adverse effects of aneuploidy are not known. To probe this question, we identified aneuploid yeast strains with improved proliferative abilities. Their molecular characterization revealed strain-specific genetic alterations as well as mutations shared between different aneuploid strains. Among the latter, a loss-of-function mutation in the gene encoding the deubiquitinating enzyme Ubp6 improves growth rates in four different aneuploid yeast strains by attenuating the changes in intracellular protein composition caused by aneuploidy. Our results demonstrate the existence of aneuploidy-tolerating mutations that improve the
fitness of multiple different aneuploidies and highlight the importance of ubiquitin-proteasomal degradation in suppressing the adverse effects of aneuploidy.
 
Overall design This dataset contains both expression analysis and CGH of yeast strains bearing extra chromosomes. In all cases, the wt euploid strain grown under the same conditions was used as the reference sample. Reference nucleic acid was generally labeled with Cy3, though some were labeled with Cy5 as indicated in the associated annotations for each array. No replicate arrays are included.

Expression Samples: GSM513249-GSM513277
CGH Samples: GSM513278-GSM513369
 
Contributor(s) Torres EM, Dephoure N, Panneerselvam A, Tucker C, Whittaker C, Gygi SP, Dunham MJ, Amon A
Citation(s) 20850176
Submission date Feb 22, 2010
Last update date Sep 02, 2016
Contact name Maitreya J. Dunham
E-mail(s) maitreya@uw.edu
Phone 206-543-2338
Organization name University of Washington
Department Genome Sciences
Lab Dunham Lab
Street address Foege Building, S403B, Box 355065
City Seattle
State/province WA
ZIP/Postal code 98195-5065
Country USA
 
Platforms (2)
GPL7542 Agilent-015072 Yeast Oligo Microarray 4x44K G2519F (Feature Number version)
GPL10045 Agilent Yeast 8X15K conversion (Agilent-016322)
Samples (121)
GSM513249 0 WT batch RNA
GSM513250 0 Dis V batch RNA
GSM513251 0 Dis V-14.1 batch RNA
Relations
BioProject PRJNA125127

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE20464_RAW.tar 804.7 Mb (http)(custom) TAR (of TXT)
GSE20464_protocols.zip.gz 362.4 Kb (ftp)(http) ZIP
Processed data included within Sample table

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