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| Status |
Public on Jul 07, 2022 |
| Title |
Auxin biosynthesis drives developmental reprogramming of differentiated cells |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Plant cells exhibit remarkable plasticity of their differentiation states, enabling regeneration of whole plants from differentiated somatic cells. How they revert cell fate and express pluripotency, however, remains unclear. In this study we demonstrate that transcriptional activation of auxin biosynthesis is crucial for reprogramming differentiated Arabidopsis leaf cells. We demonstrate that interfering with the activity of histone acetyltransferases dramatically reduces callus formation from leaf mesophyll protoplasts. Histone acetylation permits the transcriptional activation of PLETHORAs (PLTs), leading to the induction of their downstream target gene YUCCA1 (YUC1) encoding an enzyme for auxin biosynthesis. Auxin biosynthesis is in turn required to accomplish initial cell division through the activation of G2/M phase genes mediated by MYB DOMAIN PROTEIN 3-RELATED (MYB3Rs). We further show that the AUXIN RESPONSE FACTOR 7 (ARF7)/ARF19 and INDOLE-3-ACETIC ACID INDUCIBLE 3 (IAA3)/IAA18-mediated auxin signaling pathway is responsible for cell cycle reactivation by transcriptionally upregulating MYB3R4. These findings provide a mechanistic model of how differentiated plant cells revert their fate and reinitiate the cell cycle to become pluripotent.
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| Overall design |
Expression profiling for fresh and cultured mesophyll protoplasts from Arabidopsis leaves
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| |
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| Contributor(s) |
Sakamoto Y, Sugimoto K |
| Citation(s) |
35922895 |
| |
| Submission date |
May 25, 2022 |
| Last update date |
Oct 06, 2022 |
| Contact name |
Yuki Sakamoto |
| E-mail(s) |
yuki-sakamoto258@g.ecc.u-tokyo.ac.jp
|
| Phone |
+81 45 503 9575
|
| Organization name |
RIKEN
|
| Department |
CSRS
|
| Lab |
Cell Function Research Team
|
| Street address |
1-7-22, Suehiro-cho
|
| City |
Tsurumi-ku, Yokohama |
| State/province |
Kanagawa |
| ZIP/Postal code |
230-0045 |
| Country |
Japan |
| |
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| Platforms (1) |
| GPL19580 |
Illumina NextSeq 500 (Arabidopsis thaliana) |
|
| Samples (18)
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| Relations |
| BioProject |
PRJNA842228 |
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