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Series GSE204767 Query DataSets for GSE204767
Status Public on Jul 07, 2022
Title Auxin biosynthesis drives developmental reprogramming of differentiated cells
Organism Arabidopsis thaliana
Experiment type Expression profiling by high throughput sequencing
Summary Plant cells exhibit remarkable plasticity of their differentiation states, enabling regeneration of whole plants from differentiated somatic cells. How they revert cell fate and express pluripotency, however, remains unclear. In this study we demonstrate that transcriptional activation of auxin biosynthesis is crucial for reprogramming differentiated Arabidopsis leaf cells. We demonstrate that interfering with the activity of histone acetyltransferases dramatically reduces callus formation from leaf mesophyll protoplasts. Histone acetylation permits the transcriptional activation of PLETHORAs (PLTs), leading to the induction of their downstream target gene YUCCA1 (YUC1) encoding an enzyme for auxin biosynthesis. Auxin biosynthesis is in turn required to accomplish initial cell division through the activation of G2/M phase genes mediated by MYB DOMAIN PROTEIN 3-RELATED (MYB3Rs). We further show that the AUXIN RESPONSE FACTOR 7 (ARF7)/ARF19 and INDOLE-3-ACETIC ACID INDUCIBLE 3 (IAA3)/IAA18-mediated auxin signaling pathway is responsible for cell cycle reactivation by transcriptionally upregulating MYB3R4. These findings provide a mechanistic model of how differentiated plant cells revert their fate and reinitiate the cell cycle to become pluripotent.
Overall design Expression profiling for fresh and cultured mesophyll protoplasts from Arabidopsis leaves
Contributor(s) Sakamoto Y, Sugimoto K
Citation(s) 35922895
Submission date May 25, 2022
Last update date Oct 06, 2022
Contact name Yuki Sakamoto
Phone +81 45 503 9575
Organization name RIKEN
Department CSRS
Lab Cell Function Research Team
Street address 1-7-22, Suehiro-cho
City Tsurumi-ku, Yokohama
State/province Kanagawa
ZIP/Postal code 230-0045
Country Japan
Platforms (1)
GPL19580 Illumina NextSeq 500 (Arabidopsis thaliana)
Samples (18)
GSM6190787 Day 0 WT replicate 1
GSM6190788 Day 0 WT replicate 2
GSM6190789 Day 0 WT replicate 3
BioProject PRJNA842228

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Supplementary file Size Download File type/resource
GSE204767_RAW.tar 2.2 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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