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Status |
Public on Mar 03, 2023 |
Title |
Transcriptomic analysis of hepatocyte-like cells generated by doxycycline inducible expression of HNF4A, HNF1A and FOXA3 |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
We have generated an immortal fibroblast-derived cell line without malignant traits capable of being efficiently and easily reprogrammable into hepatocyte-like cells under standard culture conditions by combining hTERT expression and a polycistronic vector inducible with doxycycline.
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Overall design |
Human fibroblasts were immortalized with hTERT and infected with a lentiviral vector containing a polycistronic 2A cassette expressing HNF4A, HNF1A and FOXA3 under the control of Tetracycline Response Elements (iHDF-T). After single cell cloning, we selected a specific clone named P3G4. This clone was expanded for more than 80 population doublings (PD). We reprogrammed by doxycycline addition, low and high PD iHDF-T into hepatocyte-like cells (diHLC-T). RNA from PD low and high iHDF-T as well as diHLC-T were analyzed by RNAseq in triplicates. RNA isolated from 24hr-cultured primary hepatocytes from three different donors were used as controls.
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Contributor(s) |
Garcia-Llorens G, Pareja E, Bort R |
Citation(s) |
37072803 |
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Submission date |
May 26, 2022 |
Last update date |
Jun 05, 2023 |
Contact name |
Roque Bort |
Organization name |
IIS La Fe
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Department |
Unidad Hepatologia Experimental
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Street address |
Campanar 21
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City |
Valencia |
ZIP/Postal code |
46009 |
Country |
Spain |
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Platforms (1) |
GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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Samples (15)
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Relations |
BioProject |
PRJNA842656 |