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Series GSE204867 Query DataSets for GSE204867
Status Public on Mar 03, 2023
Title Transcriptomic analysis of hepatocyte-like cells generated by doxycycline inducible expression of HNF4A, HNF1A and FOXA3
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We have generated an immortal fibroblast-derived cell line without malignant traits capable of being efficiently and easily reprogrammable into hepatocyte-like cells under standard culture conditions by combining hTERT expression and a polycistronic vector inducible with doxycycline.
Overall design Human fibroblasts were immortalized with hTERT and infected with a lentiviral vector containing a polycistronic 2A cassette expressing HNF4A, HNF1A and FOXA3 under the control of Tetracycline Response Elements (iHDF-T).
After single cell cloning, we selected a specific clone named P3G4. This clone was expanded for more than 80 population doublings (PD). We reprogrammed by doxycycline addition, low and high PD iHDF-T into hepatocyte-like cells (diHLC-T).
RNA from PD low and high iHDF-T as well as diHLC-T were analyzed by RNAseq in triplicates. RNA isolated from 24hr-cultured primary hepatocytes from three different donors were used as controls.
Contributor(s) Garcia-Llorens G, Pareja E, Bort R
Citation(s) 37072803
Submission date May 26, 2022
Last update date Jun 05, 2023
Contact name Roque Bort
Organization name IIS La Fe
Department Unidad Hepatologia Experimental
Street address Campanar 21
City Valencia
ZIP/Postal code 46009
Country Spain
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (15)
GSM6199130 PDhigh iHDF-T_1
GSM6199131 PDhigh iHDF-T_2
GSM6199132 PDhigh iHDF-T_3
BioProject PRJNA842656

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Supplementary file Size Download File type/resource
GSE204867_count_genes.xlsx 10.1 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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