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| Status |
Public on May 31, 2022 |
| Title |
Regulation of erythropoiesis in the spleen by Desert Hedgehog (Dhh) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Dhh negatively regulates multiple stages of erythrocyte differentiation. In Dhh-deficient bone marrow, the common myeloid progenitor (CMP) population was increased, but differentiation from CMP to granulocyte/macrophage progenitor was decreased, and the mature granulocyte population was decreased, compared with wild-type (WT). In contrast, differentiation from CMP to megakaryocyte/erythrocyte progenitor was increased, and the megakaryocyte/erythrocyte progenitor population was increased. In Dhh-deficient spleen and bone marrow, BFU-Es and erythroblast populations were increased compared with WT. During recovery of hematopoiesis after irradiation, and under conditions of stress-induced erythropoiesis, erythrocyte differentiation was accelerated in both spleen and bone marrow of Dhh-deficient mice compared with WT. To investigate possible mechanisms for its regulation of erythropoiesis we carried out RNAsequencing on Facs-sorted erythroblast population II (CD71+Ter119+) cells from Dhh-/-, Dhh+/- and WR mice.
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| Overall design |
Splenocyte suspensions were prepared from 4 week old Wildtype (WT), Dhh heterozygous (Dhh+/-) and Dhh knockout (Dhh-/-) mice spleen. Cells were stained against Ter119 and CD71 and facs sorted to purify CD71+Ter119+ cells. RNA was extracted from this CD71+Ter119+ cells defined as erythroblast population II.
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| Contributor(s) |
Crompton T, Norris S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
May 28, 2022 |
| Last update date |
May 31, 2022 |
| Contact name |
Tessa Crompton |
| Organization name |
UCL
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| Department |
Immunobiology
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| Street address |
30 Guilford Street
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| City |
London |
| ZIP/Postal code |
WC1N 1EH |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA843316 |
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