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Series GSE205041 Query DataSets for GSE205041
Status Public on Aug 16, 2022
Title The mismatch-repair proteins MSH2 and MSH6 interact with the imprinting control regions through the ZFP57-KAP1 complex (ChIP-Seq)
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Genomic imprinting is controlled by CpG-rich regions (ICRs) acquiring differential methylation in the female and male germline and maintaining it in a parental origin-specific manner in somatic cells. Despite their expected mutation rate due to spontaneous deamination of methylated cytosines, ICRs maintain their CpG-richness and show conservation of CpG-bearing transcription binding sites in mammals. In order to gain further insights into the mechanisms of maintenance of methyl CpGs, we sought to identify the proteins interacting with the methylated allele of the ICRs, by determining the interactors of ZFP57 that recognizes a specific methylated ICR motif in mouse ESCs. Several proteins including factors involved in mRNA processing/splicing, chromatin organization, transcription and DNA repair were identified through a tagged approach coupled to LC–MS/MS analysis. The demonstration of the components of the post-replicative mismatch-repair (MMR) complex as ZFP57 interactors prompted us to investigate the DNA binding profile of MSH2 and MSH6 by chromatin immunoprecipitation and sequencing. We found that these proteins were enriched at the methylated allele of the ICRs and their binding was mediated by the ZFP57/KAP1 complex, suggesting that the MMR complex is recruited to these loci to preserve their genetic integrity.
Overall design In this study we perfomed Chromatin immunoprecipitation and sequencing (ChIP-seq) of Msh2 and Msh6 and methylation analysis (RRBS) in mESCs.
Contributor(s) Acurzio B, Cecere F, Giaccari C, Verma A, Angelini C, Riccio A
Citation(s) 35918739
Submission date May 28, 2022
Last update date Aug 17, 2022
Contact name Francesco Cecere
Phone 3888903265
Organization name University of Campania "Luigi Vanvitelli"
Department DiStABiF
Lab Riccio
Street address Via A. Vivaldi, n. 43
City Caserta
State/province Caserta
ZIP/Postal code 81100
Country Italy
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (6)
GSM6204474 Msh2_rep1
GSM6204475 Msh2_rep2
GSM6204476 Msh2_control
This SubSeries is part of SuperSeries:
GSE205043 The mismatch-repair proteins MSH2 and MSH6 interact with the imprinting control regions through the ZFP57-KAP1 complex
BioProject PRJNA843327

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Supplementary file Size Download File type/resource
GSE205041_RAW.tar 1.8 Gb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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