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| Status |
Public on Apr 21, 2023 |
| Title |
CTCF facilitates subset-specific chromatin interactions to limit the formation of memory CD8+ T cells [HiC] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
CD8+ T cells provide protection from infection and tumor growth, and many current immunotherapies target molecules that enhance CD8+ T cell function and differentiation. The transcriptional programs orchestrating CD8+ T cell differentiation in response to infection has been described, but the changes in spatial chromatin organization accompanying effector and memory CD8+ T cell differentiation is unknown, despite research showing the importance of long-range chromatin interactions for transcriptional regulation in various cell types. Here, we studied how genome organization is integrated with CD8+ T cell differentiation and investigated the role of CTCF, a key factor that regulates genome organization through blocking or facilitating chromatin interactions, in regulating CD8+ T cell fates. We observed T cell subset-specific changes in chromatin organization and CTCF binding, and revealed weak-affinity CTCF binding is needed to promote terminal differentiation of CD8+ T cells by coordinating chromatin interactions that regulate transcriptional programs driving CD8+ T cell differentiation.
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| Overall design |
Sorted OT-I cells day 0 or 7 days post infection with Lm-OVA
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| Contributor(s) |
Quon S, Yu B, Goldrath AW |
| Citation(s) |
37040762 |
| |
| Submission date |
May 30, 2022 |
| Last update date |
Jul 21, 2023 |
| Contact name |
Ananda Goldrath |
| E-mail(s) |
agoldrath@ucsd.edu
|
| Organization name |
UCSD
|
| Street address |
9500 Gilman Drive
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093 |
| Country |
USA |
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| Platforms (1) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE205083 |
DNA architectural protein CTCF facilitates subset-specific chromatin interactions to limit the formation of memory CD8+ T cells |
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| Relations |
| BioProject |
PRJNA843690 |
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