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Series GSE205081 Query DataSets for GSE205081
Status Public on Apr 21, 2023
Title CTCF facilitates subset-specific chromatin interactions to limit the formation of memory CD8+ T cells [HiC]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary CD8+ T cells provide protection from infection and tumor growth, and many current immunotherapies target molecules that enhance CD8+ T cell function and differentiation. The transcriptional programs orchestrating CD8+ T cell differentiation in response to infection has been described, but the changes in spatial chromatin organization accompanying effector and memory CD8+ T cell differentiation is unknown, despite research showing the importance of long-range chromatin interactions for transcriptional regulation in various cell types. Here, we studied how genome organization is integrated with CD8+ T cell differentiation and investigated the role of CTCF, a key factor that regulates genome organization through blocking or facilitating chromatin interactions, in regulating CD8+ T cell fates. We observed T cell subset-specific changes in chromatin organization and CTCF binding, and revealed weak-affinity CTCF binding is needed to promote terminal differentiation of CD8+ T cells by coordinating chromatin interactions that regulate transcriptional programs driving CD8+ T cell differentiation.
Overall design Sorted OT-I cells day 0 or 7 days post infection with Lm-OVA
Contributor(s) Quon S, Yu B, Goldrath AW
Citation(s) 37040762
Submission date May 30, 2022
Last update date Jul 21, 2023
Contact name Ananda Goldrath
Organization name UCSD
Street address 9500 Gilman Drive
City La Jolla
State/province CA
ZIP/Postal code 92093
Country USA
Platforms (1)
GPL30215 MGISEQ-2000RS (Mus musculus)
Samples (6)
GSM6204991 T8.MP.Spl1.HiC
GSM6204992 T8.MP.Spl2.HiC
GSM6204993 T8.TE.Spl1.HiC
This SubSeries is part of SuperSeries:
GSE205083 DNA architectural protein CTCF facilitates subset-specific chromatin interactions to limit the formation of memory CD8+ T cells
BioProject PRJNA843690

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Supplementary file Size Download File type/resource 9.4 Mb (ftp)(http) COOL 9.4 Mb (ftp)(http) COOL 9.4 Mb (ftp)(http) COOL
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