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Series GSE205692 Query DataSets for GSE205692
Status Public on Apr 05, 2023
Title Escape From Oncogene-Induced Senescence is Controlled by POU2F2 and Memorized by Chromatin Scars [Expression]
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Although oncogene-induced senescence (OIS) is a potent tumor-suppressor mechanism, recent studies revealed that cells can escape from OIS with features of transformed cells. However, the mechanisms that promote OIS escape remain unclear, and evidence of post-senescent cells in human cancers is missing. Here, we unravel the regulatory mechanisms underlying OIS escape using dynamic multidimensional profiling. We demonstrate a critical role for AP1 and POU2F2 transcription factors for escape from OIS and identify ‘senescence-associated chromatin scars (SACS)’ as an epigenetic memory of OIS, detectable during colorectal cancer progression. POU2F2 levels are elevated already in precancerous lesions and as cells escape from OIS, and its expression and binding activity to cis-regulatory elements are associated with decreased patient survival. Our results support a model in which POU2F2 exploits a precoded enhancer landscape to promote senescence escape and reveal POU2F2 gene signatures and SACS as valuable biomarkers with diagnostic and prognostic potential.
 
Overall design RNA from each time-point (days 0, 8, 13 , 14, 18, 19, 21, 22, 23 , 25, 32, 45, 46, 50, 56) from GM21 fibroblasts undergoing escape from RAS-induced senescence was purified using the Macherey-Nagel Nucleospin RNA Plus XS kit according to the manufacturer’s instructions. 100 ng RNA per sample was analyzed using Affymetrix Human Transcriptome Arrays 2.0
 
Contributor(s) Zamudio RI, Stefa A, Nabuco JA, Vasilopoulos T, Simpson M, Doré G, Roux P, Galan MA, Chokshi RJ, Bischof O, Herbig U
Citation(s) 37082139
Submission date Jun 08, 2022
Last update date May 12, 2023
Contact name Utz Herbig
E-mail(s) herbigut@njms.rutgers.edu
Organization name Rutgers Biomedical and Health Sciences|Rutgers University
Department Center for Cell Signaling|Rutgers‐New Jersey Medical School
Street address 205 South Orange Avenue
City Newark
State/province New Jersey
ZIP/Postal code 07103
Country USA
 
Platforms (1)
GPL17586 [HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version]
Samples (34)
GSM6217436 GM21 fibroblasts, empty vector, biol rep 1, day 32
GSM6217439 GM21 fibroblasts, empty vector, biol rep 1, day 56
GSM6217443 GM21 fibroblasts, empty vector, biol rep 1, day 8
This SubSeries is part of SuperSeries:
GSE206496 Escape From Oncogene-Induced Senescence is Controlled by POU2F2 and Memorized by Chromatin Scars
Relations
BioProject PRJNA847199

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE205692_RAW.tar 801.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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