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Series GSE205702 Query DataSets for GSE205702
Status Public on Jun 30, 2023
Title Single cell Transcriptomic analysis of CD8+ T cells treated with anti-CD137 agonists within VK12653 Multiple Myeloma bone marrow microenvironment .
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We report the single cell RNA sequencing of mouse CD8+ T cells infiltrating VK12653 Multiple Myeloma lesions upon treatment with anti-CD137 agonists .
Overall design C57BL/6 mice were injected with Vk12653 MM cells. After 10 days, mice were treated with anti-CD137 (100 µg, 3H3, BioXcell, twice a week) or with control IgG (100 µg, HRPN,BioXcell). At the indicated time points, CD8+ T cells were isolated from the BM using mouse CD8+ T cell isolation kit (Miltenyi Biotec) and FACSaria™ Fusion (BD biosciences). Single-cell libraries (3′ gene expression) were generated using the Chromium Controller Instrument and Chromium Single Cell 3′ Library & Gel Bead Kit v3 according to the manufacturer’s protocol (10× Genomics). Single-cell library size and quality were confirmed on the Fragment Analyzer (Agilent). KAPA Quantification Kit for Illumina platforms (KAPA Biosystems, Roche) was used to quantify libraries. The libraries were sequenced on a NextSeq 550 (Illumina) in pair-end sequencing 28 bp (read1) × 91 bp (read2) and a single index 8 bp in length.
Contributor(s) Martinet L, Lucca L
Citation(s) 37392737
Submission date Jun 08, 2022
Last update date Oct 03, 2023
Contact name Liliana Elisa Lucca
Organization name CRCT
Department Immunology
Street address 2 Avenue Hubert Curien
City Toulouse
ZIP/Postal code 31037
Country France
Platforms (1)
GPL21626 NextSeq 550 (Mus musculus)
Samples (10)
GSM6217568 D0-NI
GSM6217569 D0-PBS
GSM6217570 D1-CD137
BioProject PRJNA847213

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Supplementary file Size Download File type/resource
GSE205702_aCD137_expression_matrix.tsv.gz 119.5 Mb (ftp)(http) TSV
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