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| Status |
Public on Jun 30, 2023 |
| Title |
Single cell Transcriptomic analysis of CD8+ T cells treated with anti-CD137 agonists within VK12653 Multiple Myeloma bone marrow microenvironment . |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We report the single cell RNA sequencing of mouse CD8+ T cells infiltrating VK12653 Multiple Myeloma lesions upon treatment with anti-CD137 agonists .
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| Overall design |
C57BL/6 mice were injected with Vk12653 MM cells. After 10 days, mice were treated with anti-CD137 (100 µg, 3H3, BioXcell, twice a week) or with control IgG (100 µg, HRPN,BioXcell). At the indicated time points, CD8+ T cells were isolated from the BM using mouse CD8+ T cell isolation kit (Miltenyi Biotec) and FACSaria™ Fusion (BD biosciences). Single-cell libraries (3′ gene expression) were generated using the Chromium Controller Instrument and Chromium Single Cell 3′ Library & Gel Bead Kit v3 according to the manufacturer’s protocol (10× Genomics). Single-cell library size and quality were confirmed on the Fragment Analyzer (Agilent). KAPA Quantification Kit for Illumina platforms (KAPA Biosystems, Roche) was used to quantify libraries. The libraries were sequenced on a NextSeq 550 (Illumina) in pair-end sequencing 28 bp (read1) × 91 bp (read2) and a single index 8 bp in length.
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| Contributor(s) |
Martinet L, Lucca L |
| Citation(s) |
37392737 |
| |
| Submission date |
Jun 08, 2022 |
| Last update date |
Oct 03, 2023 |
| Contact name |
Liliana Elisa Lucca |
| E-mail(s) |
liliana.lucca@inserm.fr
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| Organization name |
CRCT
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| Department |
Immunology
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| Lab |
GENIM
|
| Street address |
2 Avenue Hubert Curien
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| City |
Toulouse |
| ZIP/Postal code |
31037 |
| Country |
France |
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| Platforms (1) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA847213 |
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