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Series GSE205751 Query DataSets for GSE205751
Status Public on Sep 27, 2023
Title Increased expression of LOC100506314 in T cells from patients with vitiligo and contributed to the pathogenesis of vitiligo
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The aim of this study was to investigate the differential expression of long non-coding RNAs (lncRNAs) in T cells from patients with vitiligo and their roles in the pathogenesis of vitiligo. The expression profiles of the RNA transcripts in T cells from three patients with vitiligo and three controls were conducted using microarray analysis. These aberrantly-expressed genes were further validated using T cells from 41 patients with vitiligo and 28 controls. The biologic function of the specific lncRNAs was investigated using transfection, RNA pull-down assay plus proteomic approach and Western blotting. As the results, the expression levels of 134 genes, were significantly increased, whereas the expression levels 142 genes were significantly decreased in T cells from patients with vitiligo compared with the controls. After selection and validation, the expression levels of 11 genes increased and two genes decreased in T cells from patients with vitiligo. We confirmed that LOC100506314 could interact with the signal transducer and activator of transcription 3 (STAT3) and macrophage migration inhibitory factor (MIF). The transfection of LOC100506314 could suppress STAT3, AKT, and ERK phosphorylation and nuclear protein levels of p65. Finally, overexpressed LOC100506314 decreased the T cell expression of IL-6 and IL-17. In conclusion, among the lncRNAs, we found that the expression levels of LOC100506314 were increased in T cells from patients with vitiligo. LOC100506314 could bind to STAT3 and MIF and suppress the expression of IL-6 and IL-17 through the inhibition of the STAT3, AKT, ERK, and NF-κB pathway. Increased the expression of LOC100506314 in T cells could be a potential therapeutic strategy for vitiligo.
Overall design The T cells isolation from three Vitilogo patinets PBMC and three heathly controls PBMC
Contributor(s) Yu H, Lu M
Citation(s) 37731844
Submission date Jun 09, 2022
Last update date Sep 28, 2023
Contact name Hui Chun Yu
Phone +886920179628
Organization name Dalin Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation
Department Medical research
Street address No. 2, Min-Sheng Rd, Dalin Town
City Chia-Yi
ZIP/Postal code 62247
Country Taiwan
Platforms (1)
GPL21185 Agilent-072363 SurePrint G3 Human GE v3 8x60K Microarray 039494 [Probe Name Version]
Samples (6)
GSM6222688 Control 2
GSM6222689 Control 3
GSM6222690 Control 4
BioProject PRJNA847448

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE205751_RAW.tar 74.0 Mb (http)(custom) TAR (of TXT)
GSE205751_complete_data.txt.gz 9.8 Mb (ftp)(http) TXT
Processed data included within Sample table

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