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| Status |
Public on Sep 27, 2023 |
| Title |
Increased expression of LOC100506314 in T cells from patients with vitiligo and contributed to the pathogenesis of vitiligo |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The aim of this study was to investigate the differential expression of long non-coding RNAs (lncRNAs) in T cells from patients with vitiligo and their roles in the pathogenesis of vitiligo. The expression profiles of the RNA transcripts in T cells from three patients with vitiligo and three controls were conducted using microarray analysis. These aberrantly-expressed genes were further validated using T cells from 41 patients with vitiligo and 28 controls. The biologic function of the specific lncRNAs was investigated using transfection, RNA pull-down assay plus proteomic approach and Western blotting. As the results, the expression levels of 134 genes, were significantly increased, whereas the expression levels 142 genes were significantly decreased in T cells from patients with vitiligo compared with the controls. After selection and validation, the expression levels of 11 genes increased and two genes decreased in T cells from patients with vitiligo. We confirmed that LOC100506314 could interact with the signal transducer and activator of transcription 3 (STAT3) and macrophage migration inhibitory factor (MIF). The transfection of LOC100506314 could suppress STAT3, AKT, and ERK phosphorylation and nuclear protein levels of p65. Finally, overexpressed LOC100506314 decreased the T cell expression of IL-6 and IL-17. In conclusion, among the lncRNAs, we found that the expression levels of LOC100506314 were increased in T cells from patients with vitiligo. LOC100506314 could bind to STAT3 and MIF and suppress the expression of IL-6 and IL-17 through the inhibition of the STAT3, AKT, ERK, and NF-κB pathway. Increased the expression of LOC100506314 in T cells could be a potential therapeutic strategy for vitiligo.
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| Overall design |
The T cells isolation from three Vitilogo patinets PBMC and three heathly controls PBMC
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| Contributor(s) |
Yu H, Lu M |
| Citation(s) |
37731844 |
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| Submission date |
Jun 09, 2022 |
| Last update date |
Sep 28, 2023 |
| Contact name |
Hui Chun Yu |
| E-mail(s) |
junvsusagi@gmail.com
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| Phone |
+886920179628
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| Organization name |
Dalin Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation
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| Department |
Medical research
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| Street address |
No. 2, Min-Sheng Rd, Dalin Town
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| City |
Chia-Yi |
| ZIP/Postal code |
62247 |
| Country |
Taiwan |
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| Platforms (1) |
| GPL21185 |
Agilent-072363 SurePrint G3 Human GE v3 8x60K Microarray 039494 [Probe Name Version] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA847448 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE205751_RAW.tar |
74.0 Mb |
(http)(custom) |
TAR (of TXT) |
| GSE205751_complete_data.txt.gz |
9.8 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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