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Series GSE206120 Query DataSets for GSE206120
Status Public on Jan 23, 2023
Title Engraftment of allogeneic iPS cell-derived cartilage organoid in a primate model of articular cartilage defect
Organism Macaca fascicularis
Experiment type Expression profiling by high throughput sequencing
Summary Induced pluripotent stem cells (iPSCs) are a promising resource for allogeneic cartilage transplantation to treat articular cartilage defects that do not heal spontaneously and often progress to debilitating conditions, such as osteoarthritis. However, to the best of our knowledge, allogeneic cartilage transplantation into primate models has never been assessed. Here, we show that allogeneic iPSC-derived cartilage organoids survive and integrate as well as function as articular cartilage in a primate model of chondral defects in the knee joints. Histological analysis revealed that allogeneic iPSC-derived cartilage organoids in chondral defects elicited no immune reaction and directly contributed to the tissue repair for at least four months. iPSC-derived cartilage organoids integrated with the host native articular cartilage and prevented degeneration of the surrounding cartilage. Single-cell RNA-sequence analysis indicated that iPSC-derived cartilage organoids differentiated after transplantation, acquiring expression of PRG4 that is crucial for joint lubrication. Pathway analysis suggested the involvement of SIK3 inactivation, verified through molecular experiments. Our study outcomes suggest that allogeneic transplantation of iPSC-derived cartilage organoids may be clinically applicable for the treatment of patients with chondral defects of the articular cartilage.
 
Overall design We performed a single cell (sc)RNA-seq transcriptome analysis of cynomolgus monkey iPS cells (cyiPSC) and cyiPSC-derived cartilage (pre-transplant cyiPS-Cart). We created chondral defects in articular cartilage in knee joints of cynomolgus monkeys. We transplanted allogeneic cyiPS-Cart or nothing. Four months after transplantation, articular cartilage (AC), transplanted cyiPS-Cart (post-transplanted cyiPS-Cart) and fibrous tissue (cyFT) formed in the chondral defects where nothing had been transplanted were harvested and subjected to scRNA-seq analysis.
 
Contributor(s) Abe K, Koyamatsu S, Tsumaki N
Citation(s) 36808132
Submission date Jun 14, 2022
Last update date Mar 15, 2023
Contact name Noriyuki Tsumaki
E-mail(s) tsumaki.noriyuki.med@osaka-u.ac.jp
Phone +81668793321
Organization name Osaka University, Graduate School of Medicine
Department Department of Biochemistry and Molecular Biology
Lab Tissue Biochemistry
Street address 2-2 Yamadaoka
City Suita
State/province Select One
ZIP/Postal code 565-0871
Country Japan
 
Platforms (1)
GPL28212 Illumina NovaSeq 6000 (Macaca fascicularis)
Samples (6)
GSM6243611 WTA12: cyiPSCs and pre-transplant cyiPS-Cart
GSM6243612 Tag12: cyiPSCs and pre-transplant cyiPS-Cart
GSM6243613 WTA15: post-transplant cyiPS-Cart
Relations
BioProject PRJNA849238

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE206120_Fig5.matrix.txt.gz 35.1 Mb (ftp)(http) TXT
GSE206120_Fig6.matrix.txt.gz 5.0 Mb (ftp)(http) TXT
GSE206120_Supplemental_table.xlsx 10.7 Kb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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