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Series GSE206507 Query DataSets for GSE206507
Status Public on Nov 14, 2022
Title Tissue adaptation and clonal segregation of human memory T cells in barrier sites
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary T lymphocytes migrate to barrier sites after exposure to pathogen-derived antigens to provide localized immune defense and long-term surveillance. However, unique tissue adaptations of barrier T cells and the extent to which they participate in clonal networks remains unclear. In this study, we utilized our organ donor tissue resource to examine T cells in barrier sites (skin, lung, jejunum), their associated lymph nodes, other lymphoid tissues (spleen and bone marrow), and circulation. By integrating multiple single-cell protein and transcript profiling technologies, we demonstrate that human barrier sites contain site-adapted memory T cell populations, including tissue-resident memory T cells (TRM) that exhibit both shared barrier TRM signatures and tissue-specific residency profiles. Additionally, incorporating T cell receptor and RNA-sequencing analysis, we show that lung T cell clones form global networks with clones in lymphoid sites and circulation, and comprise widely disseminated clones of TEM/TEMRA subset that display distinct effector phenotypes; while T cells in skin and jejunum are predominantly TRM and participate primarily in localized clonal networks with tissue-associated lymph nodes but are largely compartmentalized.
Overall design joint single-cell RNA/T cell receptor sequencing
Contributor(s) Poon MM, Caron D, Wang Z, Sims PA, Shen Y, Farber DL
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Submission date Jun 20, 2022
Last update date Nov 14, 2022
Contact name Peter A Sims
Organization name Columbia University
Street address 3960 Broadway, Lasker 203AC
City New York
State/province NY
ZIP/Postal code 10032
Country USA
Platforms (2)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (32)
GSM6255587 D492 GEX lung
GSM6255588 D492 GEX bone marrow
GSM6255589 D492 GEX skin
BioProject PRJNA851108

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Supplementary file Size Download File type/resource
GSE206507_D492_D511.gex.matrix.txt.gz 86.1 Mb (ftp)(http) TXT
GSE206507_D492_D511.tcr.matrix.txt.gz 947.5 Kb (ftp)(http) TXT
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