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Series GSE206968 Query DataSets for GSE206968
Status Public on Jun 09, 2023
Title N6-methyladenosine-enhanced FZR1 translation contributes to gemcitabine insensitivity in pancreatic cancer via quiescence maintenance [m6A-seq]
Organism Homo sapiens
Experiment type Methylation profiling by high throughput sequencing
Summary Herein, by detecting the global m6A profile in a panel of gemcitabine-sensitive and gemcitabine-insensitive PDAC cells, we identified the key role of hyper-m6A modification of the master G0/G1 regulator Fizzy and Cell Division Cycle 20 Related 1 (FZR1) in regulating gemcitabine sensitivity. Targeting m6A modification of FZR1 improves the gemcitabine treatment response of gemcitabine-insensitive PDAC cells in vitro and in vivo.
Mechanistically, Gem Nuclear Organelle Associated Protein 5 (GEMIN5) was identified as a novel m6A mediator that specifically binds to m6A-modified FZR1 and recruits the eIF3 translation initiation complex to accelerate FZR1 translation. Upregulation of FZR1 maintains the G0/G1 quiescent state and impairs gemcitabine sensitivity of PDAC cells. Further clinical analysis showed that both high levels of FZR1 m6A modification and FZR1 protein indicated a poor response to gemcitabine.
Overall design m6A-seq data for six PDAC cell lines and GEMIN5 PAR-CLIP-seq of PANC-1
Contributor(s) Su J, Li R
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Submission date Jun 27, 2022
Last update date Jun 11, 2023
Contact name Rui Li
Organization name Sun Yat-sen University Cancer Center
Street address 651 Dongfeng East Road
City Guangzhou
ZIP/Postal code 510060
Country China
Platforms (1)
GPL20795 HiSeq X Ten (Homo sapiens)
Samples (12)
This SubSeries is part of SuperSeries:
GSE206969 N6-methyladenosine modification of FZR1 mRNA promotes gemcitabine resistance in pancreatic cancer
BioProject PRJNA853161

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Supplementary file Size Download File type/resource
GSE206968_RAW.tar 120.0 Kb (http)(custom) TAR (of NARROWPEAK)
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Raw data are available in SRA
Processed data provided as supplementary file

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