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Series GSE207069 Query DataSets for GSE207069
Status Public on Dec 01, 2023
Title Transcriptome and chromatin changes during CRC organoid outgrowth as proxy for metastatic outgrowth [bulkRNAseq_AKPS]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Human engineered CRC organoids were equipped with the intestinal stem cell reporter STAR reflecting transcriptional activity of ASCL2. Bulk RNA-sequencing was performed on STAR populations after 5 days and after 12 days of plating single STAR+ or STAR- cells.
Overall design Organoids were grown from single STAR+ or STAR- cells (APCKO/KO; KRASG12D/-; TP53KO/KO; SMAD4 KO/KO (AKPS)). After 5 days and after 12 days, organoids were harvested, dissociated to single cells, and between 10-20 k STAR+ and STAR- cells were collected in technical replicates for bulk RNA-seq.
Contributor(s) Heinz MC, Lindeboom RG, Oost KC, van Voorthuijsen L, Vermeulen M, Snippert H
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Submission date Jun 28, 2022
Last update date Dec 01, 2023
Contact name Maria Christine Heinz
Organization name University Medical Center Utrecht
Department Molecular Cancer Research
Lab Hugo Snippert
Street address Heidelberglaan 100
City Utrecht
ZIP/Postal code 3584 CX
Country Netherlands
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (21)
GSM6276690 RNA_AKPS_Day5_STAR-neg-to-neg_1
GSM6276691 RNA_AKPS_Day5_STAR-neg-to-pos_1
GSM6276692 RNA_AKPS_Day5_STAR-neg-to-pos_2
This SubSeries is part of SuperSeries:
GSE207071 Transcriptome and chromatin changes during CRC organoid outgrowth as proxy for metastatic outgrowth
BioProject PRJNA853559

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Supplementary file Size Download File type/resource
GSE207069_RAW.tar 3.0 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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