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| Status |
Public on Dec 01, 2023 |
| Title |
Transcriptome and chromatin changes during CRC organoid outgrowth as proxy for metastatic outgrowth [bulkRNAseq_AKPS] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Human engineered CRC organoids were equipped with the intestinal stem cell reporter STAR reflecting transcriptional activity of ASCL2. Bulk RNA-sequencing was performed on STAR populations after 5 days and after 12 days of plating single STAR+ or STAR- cells.
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| Overall design |
Organoids were grown from single STAR+ or STAR- cells (APCKO/KO; KRASG12D/-; TP53KO/KO; SMAD4 KO/KO (AKPS)). After 5 days and after 12 days, organoids were harvested, dissociated to single cells, and between 10-20 k STAR+ and STAR- cells were collected in technical replicates for bulk RNA-seq.
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| Contributor(s) |
Heinz MC, Lindeboom RG, Oost KC, van Voorthuijsen L, Vermeulen M, Snippert H |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jun 28, 2022 |
| Last update date |
Dec 01, 2023 |
| Contact name |
Maria Christine Heinz |
| E-mail(s) |
Maria.Puschhof@uni-heidelberg.de
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| Organization name |
University Medical Center Utrecht
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| Department |
Molecular Cancer Research
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| Lab |
Hugo Snippert
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| Street address |
Heidelberglaan 100
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| City |
Utrecht |
| ZIP/Postal code |
3584 CX |
| Country |
Netherlands |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (21)
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| This SubSeries is part of SuperSeries: |
| GSE207071 |
Transcriptome and chromatin changes during CRC organoid outgrowth as proxy for metastatic outgrowth |
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| Relations |
| BioProject |
PRJNA853559 |
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