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Series GSE207330 Query DataSets for GSE207330
Status Public on May 05, 2023
Title Translational fidelity screens reveal eIF3 as a key regulator of start codon selectivity [RNA-seq]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary To investigate the function eIF3D, eIF1A, eIF4G2, and ZNF324 in near-cognate start codon usage, we established K562, HeLa, and Jurkat cell lines in which each target gene has been knocked down by CRISPRi sgRNAs.
Overall design We then performed RNA-seq in K562 cells depleted of all target genes and RNA-seq in HeLa and Jurkat cells depleted for eIF3D.
Contributor(s) She R, Weissman JS
Citation(s) 37144468
Submission date Jul 01, 2022
Last update date May 05, 2023
Contact name Richard She
Organization name Whitehead Institute
Lab Weissman Lab
Street address 455 Main St Rm 639, Whitehead Institute
City Cambridge
State/province Massachusetts
ZIP/Postal code 02142
Country USA
Platforms (2)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
Samples (14)
GSM6284625 RNA-seq of HeLa cells, Day 5, eIF3D sgRNA
GSM6284626 RNA-seq of HeLa cells, Day 0, WT
GSM6284627 RNA-seq of Jurkat cells, Day 5, eIF3D sgRNA
BioProject PRJNA854886

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Supplementary file Size Download File type/resource
GSE207330_K562_RNAseq_Table1.txt.gz 621.7 Kb (ftp)(http) TXT
GSE207330_K562_RNAseq_Table2.txt.gz 708.5 Kb (ftp)(http) TXT
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