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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jul 09, 2022 |
| Title |
A cellular hierarchy in melanoma uncouples growth and metastasis |
| Organisms |
Homo sapiens; Mus; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
Although melanoma is notorious for its high degree of heterogeneity and plasticity, the origin and magnitude of cell state diversity remains poorly understood. Equally, it is not known whether melanoma growth and metastatic dissemination are supported by overlapping or distinct melanoma subpopulations. By combining mouse genetics, unbiased lineage tracing and quantitative modelling, single-cell and spatial transcriptomics, we provide evidence of a hierarchical model of tumour growth that mirrors the cellular and molecular logic underlying embryonic neural crest cell fate specification and differentiation. Our findings indicate that tumorigenic competence is associated with a spatially localized perivascular niche environment, a phenotype acquired through a NOTCH3-dependent intercellular communication pathway established by endothelial cells. Consistent with a model in which only a fraction of melanoma cells is fated to fuel growth, temporal single-cell tracing of a population of melanoma cells harbouring a mesenchymal-like state revealed that these cells do not contribute to primary tumour growth but, instead, constitutes a pool of metastatic-initiating cells that can switch cell identity while disseminating to secondary organs. Our data provide a spatially and temporally resolved map of the diversity and trajectories of cancer cell states within the evolving melanoma ecosystem and suggest that the ability to support growth and metastasis are limited to distinct pools of melanoma cells. The observation that these phenotypic competencies can be dynamically acquired upon exposure to specific niche signals warrant the development of therapeutic strategies that interfere with the cancer cell reprogramming activity of such microenvironmental cues.
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| Overall design |
scRNA-Seq and bulk RNA-Seq
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| Contributor(s) |
Rambow F, Karras P, Pozniak J |
| Citation(s) |
36131018, 38963759 |
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| Submission date |
Jul 06, 2022 |
| Last update date |
Aug 26, 2024 |
| Contact name |
Panagiotis Cristophe Karras |
| E-mail(s) |
panagiotis.karras@kuleuven.vib.be
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| Phone |
0479673407
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| Organization name |
VIB Center for Cancer Biology
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| Street address |
Herestraat 49
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| City |
leuven |
| State/province |
belgium |
| ZIP/Postal code |
3000 |
| Country |
Belgium |
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| Platforms (3) |
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| Samples (25)
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| Relations |
| BioProject |
PRJNA856289 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE207592_MM047siINVTFsMatrix.txt.gz |
232.7 Kb |
(ftp)(http) |
TXT |
| GSE207592_RAW.tar |
3.6 Gb |
(http)(custom) |
TAR (of CSV, H5, MTX, TIFF, TSV, TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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