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Series GSE207982 Query DataSets for GSE207982
Status Public on Mar 17, 2023
Title NF-κB/p52 augments ETS1 binding genome-wide to promote glioma progression
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
Summary Gliomas are among the most invasive and chemo-resistant cancers, making them challenging to treat. Chronic inflammation is one of the key drivers of glioma progression as it promotes the aberrant activation of inflammatory pathways such as NF-κB signalling which drives cancer cell invasion, angiogenesis and tissue remodelling. NF-κB factors typically dimerize with its own family members, but emerging evidence of their promiscuous interactions with other oncogenic factors have been reported to activate the transcription of new target genes and function. Here, we show that non-canonical NF-κB activation directly regulates p52 at the ETS1 promoter to activate its expression. This in turn impacts the genomic and transcriptional landscape of ETS1 in a glioma-specific manner. We further show that enhanced non-canonical NF-κB signalling promotes the co-localization of p52 and ETS1, resulting in the transcriptional activation of non-κB and/or non-ETS glioma-promoting genes. We conclude that p52-induced ETS1 overexpression in glioma cells remodels the genome-wide regulatory network of p52 and ETS1 to transcriptionally drive cancer progression
 
Overall design Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for p52 and ETS1 in control and ETS1 knockdown U-87 MG cells, with and without TWEAK stimulation

Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for RNApol2 in control, ETS1 and NFKB2 knockdown U-87 MG cells, with and without TWEAK stimulation

RNA-sequencing on control untreated, control TWEAK treated, ETS1 knockdown TWEAK treated and NFKB2 knockdown TWEAK treated U87-MG cells
 
Contributor(s) Sim N, Li Y
Citation(s) 37087499
Submission date Jul 12, 2022
Last update date Apr 24, 2023
Contact name Yinghui Li
E-mail(s) liyh@ntu.edu.sg
Organization name Nanyang Technological University
Department School of Biological Sciences
Lab 05n-21
Street address 60 Nanyang Drive
City Singapore
State/province Singapore
ZIP/Postal code 637551
Country Singapore
 
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (35)
GSM6329556 U87 control input
GSM6329557 U87 ETS1 KD guide 1 input
GSM6329558 U87 ETS1 KD guide 2 input
Relations
BioProject PRJNA857900

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE207982_RAW.tar 5.8 Mb (http)(custom) TAR (of NARROWPEAK)
GSE207982_U87_C_TW_ETS_sorted_merged.bw 264.1 Mb (ftp)(http) BW
GSE207982_U87_C_TW_RNApol2_merged.bw 178.0 Mb (ftp)(http) BW
GSE207982_U87_C_UT_ETS_sorted_merged.bw 273.7 Mb (ftp)(http) BW
GSE207982_U87_C_UT_RNApol2_merged.bw 154.3 Mb (ftp)(http) BW
GSE207982_U87_C_UT_p52_sorted_merged.bw 264.4 Mb (ftp)(http) BW
GSE207982_U87_C_p52_sorted_merged.bw 246.2 Mb (ftp)(http) BW
GSE207982_U87_ETS1KD_TW_RNApol2_merged.bw 181.4 Mb (ftp)(http) BW
GSE207982_U87_Ex5_p52_sorted_merged.bw 250.6 Mb (ftp)(http) BW
GSE207982_U87_Ex7_p52_sorted_merged.bw 251.0 Mb (ftp)(http) BW
GSE207982_U87_RNAseq_counts.txt.gz 751.8 Kb (ftp)(http) TXT
GSE207982_U87_p52KD_TW_RNApol2_merged.bw 188.4 Mb (ftp)(http) BW
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record
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