|
| Status |
Public on Jan 18, 2023 |
| Title |
Revealing the small proteome of Haloferax volcanii by ribosome profiling and small-protein optimised mass spectrometry |
| Organism |
Haloferax volcanii |
| Experiment type |
Expression profiling by high throughput sequencing
Other
|
| Summary |
We present a combinatorial approach, integrating experimental data from small protein-optimized mass spectrometry (MS) and ribosome profiling (Ribo-seq), to generate a high confidence inventory of small proteins in the model archaeon Haloferax volcanii.
|
| |
|
| Overall design |
Ribo-seq and RNA-seq experiments to study the transcriptome and the translatome of H. volcanii
|
| |
|
| Contributor(s) |
Hadjeras L, Bartel J, Sharma CM, Marchfelder A |
| Citation(s) |
37223747 |
|
https://doi.org/10.1093/femsml/uqad001
|
| |
| Submission date |
Jul 13, 2022 |
| Last update date |
Aug 25, 2023 |
| Contact name |
Rick Gelhausen |
| E-mail(s) |
gelhausr@informatik.uni-freiburg.de
|
| Organization name |
Albert-Ludwigs-University Freiburg
|
| Department |
Department of Computer Science
|
| Lab |
Bioinformatics Group (AG Backofen)
|
| Street address |
Georges-Köhler-Allee 106
|
| City |
Freiburg |
| State/province |
Baden Württemberg |
| ZIP/Postal code |
79110 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL23691 |
Illumina NextSeq 500 (Haloferax volcanii) |
|
| Samples (6)
|
| GSM6336916 |
wild-type, MNase, RIBO-seq, Replicate 1 |
| GSM6336917 |
wild-type, MNase, RIBO-seq, Replicate 2 |
| GSM6336918 |
wild-type, RNaseI, RIBO-seq, Replicate 1 |
| GSM6336919 |
wild-type, MNase, RNA-seq, Replicate 1 |
| GSM6336920 |
wild-type, MNase, RNA-seq, Replicate 2 |
| GSM6336921 |
wild-type, RNaseI, RNA-seq, Replicate 1 |
|
| Relations |
| BioProject |
PRJNA858256 |
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