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Series GSE2089 Query DataSets for GSE2089
Status Public on Dec 21, 2004
Title Comparative transcriptome analysis of early interaction events in Scots pine root tissues following challenge (1dpi)
Platform organism Pinus taeda
Sample organism Pinus sylvestris
Experiment type Expression profiling by array
Summary Comparative transcriptome analysis of early interaction events in Scots pine root tissues following challenge with a pathogenic, saprophytic or symbiotic fungus. Seedlings of P. sylvestris (19 days post germination) were transferred to wet, sterile filter paper on Petri-plates. Thereafter, the roots of the seedlings were inoculated with the mycelial homogenate of either Heterobasidion annosum (FP5, P-type) a pathogenic root rot fungus which attacks Norway spruce, Scots pine and broad leaf trees or Laccaria bicolor, an obligate ectomycorrhizal symbiont or Trichoderma aureoviride- an obligate saprotroph. Thereafter, incubated for 30 minutes, during which time some hyphae adhered to the roots. The inoculated seedlings (ten) were then transferred to another wet sterile filter paper placed on 1% water agar in Petri dishes. A second set of moist sterile filter paper was laid over the roots. The region of the Petri-dish containing the roots was covered with aluminium foil and the edges of the plate sealed with parafilm. The seedlings were then incubated for 24 hr under a photoperiod of 16h light at 20 ºC. Control seedlings were ‘inoculated’ with sterile distilled water.
Keywords: other
 
 
Contributor(s) Asiegbu F, Olson A, Li G, Adomas A, Craig D, Chu T, Heller G, Karlsson M, Nahalkova J, van Zyl L, Wolfinger R, Sederoff R, Dean R, Finlay R, Stenlid J
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Submission date Dec 20, 2004
Last update date Mar 17, 2012
Contact name Debbie Ross Craig
E-mail(s) dlcraig@unity.ncsu.edu
Phone (919)513-0015
Fax (919)515-7801
Organization name North Carolina State University
Department Forestry
Lab Forest Biotechnology
Street address 840 Main Campus Drive
City Raleigh
State/province NC
ZIP/Postal code 27695
Country USA
 
Platforms (1)
GPL1508 Pinus taeda cDNA
Samples (36)
GSM37686 rep B Mycorrhiza Saprotroph (12383916)
GSM37695 rep B Mycorrhiza Saprotroph (12384450)
GSM37699 rep C Pathogen Control (12380338)
This SubSeries is part of SuperSeries:
GSE5410 Comparative transcriptome analysis of early interaction events in Scots pine root tissues following challenge
Relations
BioProject PRJNA104755

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