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Series GSE209707 Query DataSets for GSE209707
Status Public on Dec 01, 2023
Title The UBP5 histone H2A deubiquitinase counteracts PRCs-mediated repression to regulate Arabidopsis development
Organism Arabidopsis thaliana
Experiment type Expression profiling by high throughput sequencing
Summary Polycomb Repressive Complexes (PRCs) control gene expression through the incorporation of H2Aub and H3K27me3. In recent years, there is increasing evidence of the complexity of PRCs’ interaction networks and the interplay of these interactors with PRCs in epigenome reshaping, which is fundamental to understand gene regulatory mechanisms. Here, we identified UBIQUITIN SPECIFIC PROTEASE 5 (UBP5) as a chromatin player able to counteract the deposition of the two PRCs epigenetic hallmarks in Arabidopsis thaliana. We demonstrated that UBP5 is a plant developmental regulator based on functional analyses of ubp5-CRISPR Cas9 mutant plants. UBP5 promotes H2A monoubiquitination erasure, leading to transcriptional de-repression. Furthermore, preferential association of UBP5 at PRC2 recruiting motifs and local H3K27me3 gaining in ubp5 mutant plants suggest the existence of functional interplays between UBP5 and PRC2 in regulating epigenome dynamics. In summary, acting as an antagonist of the pivotal epigenetic repressive marks H2Aub and H3K27me3, UBP5 provides novel insights to disentangle the complex regulation of PRCs’ activities.
Overall design Comparison of transcriptomes of Col-0 and ubp5 mutant seedlings using 3'RNA-seq (four biological replicates).
Contributor(s) Godwin J, Krzyszton M, Swiezewski S, Farrona S
Citation(s) 38253560
Submission date Jul 25, 2022
Last update date Mar 01, 2024
Contact name Michal Krzyszton
Organization name Institute of Biochemistry and Biophysics, Polish Academy of Sciences
Lab Laboratory of Seeds Molecular Biology
Street address Pawinskiego 5a
City Warsaw
ZIP/Postal code 02-106
Country Poland
Platforms (1)
GPL24270 NextSeq 550 (Arabidopsis thaliana)
Samples (8)
GSM6384111 Col0_1
GSM6384112 Col0_2
GSM6384113 Col0_3
BioProject PRJNA862130

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE209707_RAW.tar 990.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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