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Series GSE210202 Query DataSets for GSE210202
Status Public on Oct 02, 2023
Title Loss of Feedback Regulation between FAM3B and Androgen Receptor Driving Prostate Cancer Progression
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary TMPRSS2-ERG gene fusion is one of the most frequent genetic alterations in prostate cancer (PC). Through a systematical survey of the loss of interstitial genes and patient outcomes, we nominated FAM3B as the top-ranked interstitial genes whose loss is associated with a poor prognosis. Using cultured cells, patient- and cell-line-derived xenograft models in gonad-intact or castrated host, and genetically engineered mouse models with FAM3B overexpression, knockdown, or knockout, we defined a driver role of FAM3B loss in prostate cancer progression. By performing RNA-seq analysis in two pre-clinical models with ectopic FAM3B expression or FAM3B knockdown, we identified that the androgen response pathway was commonly modulated by FAM3B in these two models. Further validation studies showed that FAM3B inhibites AR activity.
Overall design FAM3B-inducible-expressing C4-2 cells and the control cells were treated with 200 ng/ml doxycycline for 48 hours. Total RNA was isolated from these cells and castration-resistant control-shRNA- or FAM3B-shRNA-expressing VCaP tumors using the TRIzol reagent. RNA integrity was assessed using an Agilent Bioanalyzer. The RNA samples from C4-2 cells were sent to Novogene for polyA-selected, 150-base paired-end RNA sequencing on an Illumina platform, and those from the VCaP tumors were sent to the Beijing Genomics Institute for polyA-selected, 100-base paired-end stranded RNA sequencing on the DNBSEQ platform.
Contributor(s) Dong Y, Ma T
Citation(s) 37847647
Submission date Aug 01, 2022
Last update date Jan 01, 2024
Contact name Yan Dong
Organization name Tulane University
Street address 1700 Tulane Ave
City New Orleans
ZIP/Postal code 70112
Country USA
Platforms (2)
GPL9052 Illumina Genome Analyzer (Homo sapiens)
GPL29480 DNBSEQ-T7 (Homo sapiens)
Samples (12)
GSM6423447 C4-2_Dox_Ctrl_1
GSM6423448 C4-2_Dox_Ctrl_2
GSM6423449 C4-2_Dox_FAM3B_1
BioProject PRJNA864771

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Supplementary file Size Download File type/resource
GSE210202_RAW.tar 17.1 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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