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Series GSE2113 Query DataSets for GSE2113
Status Public on Feb 08, 2005
Title Plasma cell dyscrasias expression profiles associated with distinct IGH translocations in multiple myeloma
Organism Homo sapiens
Experiment type Expression profiling by array
Summary This series of microarray experiments contains the gene expression profiles of purified plasma cells (PCs) obtained from 7 monoclonal gammopathy of undetermined significance (MGUS), 39 newly diagnosed multiple myeloma (MM) and 6 plasma-cell leukaemia (PCL) patients. PCs were purified from bone marrow Seriess, after red blood cell lysis with 0.86% ammonium chloride, using CD138 immunomagnetic microbeads. The purity of the positively selected PCs was assessed by morphology and flow cytometry and was > 90% in all cases. 5 micrograms of total RNA was processed and hybridized to the Affymetrix HG-U133A chip following the manufacturer's instructions. After scanning, the images were processed using Affymetrix MicroArray Suite (MAS) 5.0 software to generate gene expression intensity values. Arrays normalization was performed using MAS 5.0 "global scaling" procedure, which normalizes the signals of different experiments to the same target intensity (TGT=100).
Keywords: other
 
 
Contributor(s) Mattioli M, Agnelli L, Fabris S, Baldini L, Morabito F, Bicciato S, Verdelli D, Daniela I, Nobili L, Cro L, Pruneri G, Callea V, Stelitano C, Maiolo A, Lombardi L, Neri A
Citation(s) 15735737
Submission date Dec 27, 2004
Last update date Aug 10, 2018
Contact name Luca Agnelli
E-mail(s) luca.agnelli@istitutotumori.mi.it, luca.agnelli@gmail.com
Phone +390223903581
Organization name IRCCS Istituto Nazionale dei Tumori
Department Department of Advanced Diagnostics
Street address Venezian 1
City MILAN
ZIP/Postal code 20133
Country Italy
 
Platforms (1)
GPL96 [HG-U133A] Affymetrix Human Genome U133A Array
Samples (52)
GSM38155 MGUS-005
GSM38156 MGUS-008
GSM38157 MGUS-015
Relations
BioProject PRJNA91771

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Supplementary file Size Download File type/resource
GSE2113_RAW.tar 176.8 Mb (http)(custom) TAR (of CEL)

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