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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Mar 27, 2023 |
| Title |
Identifying signatures of EV secretion in metastatic breast cancer through functional single-cell profiling |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Extracellular vesicles (EVs) regulate the tumor microenvironment by facilitating transport of biomolecular cargo including RNA, protein, and metabolites. We developed an integrated method to identify individual cells with differences in EV secretion and linked single-cell RNA-sequencing on cloned single cells from MDAMB231 cell line. Additionally, We profiled parental MDAMB231, MCF7 and HCC70 cell lines.
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| Overall design |
Single-cell RNA sequencing of two monoclonal cell lines (MDAMB231-S with high and MDAMB231-NS with low EV secretion), parental MDAMB231, MCF7, and HCC70 cell lines.
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| Contributor(s) |
Fathi M, Martinez-Paniagua M, Rezvan A, Martinez-Paniagua M, Rezvan A, Montalvo MJ, Mohanty V, Chen K, Mani SA, Varadarajan N |
| Citation(s) |
37091228 |
| Submission date |
Aug 18, 2022 |
| Last update date |
May 12, 2023 |
| Contact name |
Navin Varadarajan |
| E-mail(s) |
nvaradar@central.uh.edu
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| Organization name |
University of Houston
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| Department |
Chemical and Biomolecular Engineering
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| Lab |
SingleCell lab
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| Street address |
4722 Calhoun Rd
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| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77004 |
| Country |
USA |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (1) |
| GSM6477009 |
MDAMB231-S, MDAMB231-NS, MDAMB231, MCF7, and HCC70 |
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| Relations |
| BioProject |
PRJNA870910 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE211561_RAW.tar |
32.9 Mb |
(http)(custom) |
TAR (of CSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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