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| Status |
Public on Sep 02, 2022 |
| Title |
Transcriptomic profiling of cardiac tissues from SARS-CoV-2 patients identifies DNA damage |
| Organisms |
Homo sapiens; blank sample |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
In this study, we investigated the landscape of cardiac tissues collected at rapid autopsy from 7 SARS-CoV-2, 2 pH1N1, and 6 control patients using targeted spatial transcriptomics approaches. The main outcomes for the study were to profile rapid autopsy samples collected from the COVID-19, pH1N1 and normal, non-viral deaths to determine transcriptional changes between the different cohorts. Although SARS-CoV-2 was not detected in cardiac tissue, host transcriptomics showed upregulation of genes associated with DNA damage and repair, heat shock, and M1-like macrophage infiltration in the cardiac tissues of COVID-19 patients. The DNA damage present in the SARS-CoV-2 patient samples, were further confirmed by g-H2Ax immunohistochemistry. In comparison, pH1N1 showed upregulation of Interferon-stimulated genes (ISGs), in particular interferon and complement pathways, when compared with COVID-19 patients.
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| Overall design |
In this dataset, we capture GeoMx DSP RNA profiles of myocardial tissue from SARS-CoV2 infected patients aswell as pH1N1 and control specimens
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| Contributor(s) |
Kulasinghe AK, Tan CW |
| Citation(s) |
36107637 |
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| Submission date |
Aug 26, 2022 |
| Last update date |
Oct 28, 2022 |
| Contact name |
Arutha Kulasinghe |
| E-mail(s) |
arutha.kulasinghe@uq.edu.au
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| Organization name |
University of Queensland
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| Street address |
37 kent st
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| City |
brisbane |
| ZIP/Postal code |
4102 |
| Country |
Australia |
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| Platforms (2) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
| GPL29487 |
Illumina NovaSeq 6000 (blank sample) |
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| Samples (50)
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| Relations |
| BioProject |
PRJNA874043 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE212119_RAW.tar |
1.4 Mb |
(http)(custom) |
TAR (of DCC) |
| GSE212119_TMA1_QC.csv.gz |
227.8 Kb |
(ftp)(http) |
CSV |
| GSE212119_TMA1_initial.csv.gz |
223.3 Kb |
(ftp)(http) |
CSV |
| GSE212119_TMA2_QC.csv.gz |
248.1 Kb |
(ftp)(http) |
CSV |
| GSE212119_TMA2_initial.csv.gz |
247.0 Kb |
(ftp)(http) |
CSV |
| GSE212119_normalisedCountMatrix.csv.gz |
743.7 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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