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Series GSE212119 Query DataSets for GSE212119
Status Public on Sep 02, 2022
Title Transcriptomic profiling of cardiac tissues from SARS-CoV-2 patients identifies DNA damage
Organisms Homo sapiens; blank sample
Experiment type Expression profiling by high throughput sequencing
Summary In this study, we investigated the landscape of cardiac tissues collected at rapid autopsy from 7 SARS-CoV-2, 2 pH1N1, and 6 control patients using targeted spatial transcriptomics approaches. The main outcomes for the study were to profile rapid autopsy samples collected from the COVID-19, pH1N1 and normal, non-viral deaths to determine transcriptional changes between the different cohorts. Although SARS-CoV-2 was not detected in cardiac tissue, host transcriptomics showed upregulation of genes associated with DNA damage and repair, heat shock, and M1-like macrophage infiltration in the cardiac tissues of COVID-19 patients. The DNA damage present in the SARS-CoV-2 patient samples, were further confirmed by g-H2Ax immunohistochemistry. In comparison, pH1N1 showed upregulation of Interferon-stimulated genes (ISGs), in particular interferon and complement pathways, when compared with COVID-19 patients.
Overall design In this dataset, we capture GeoMx DSP RNA profiles of myocardial tissue from SARS-CoV2 infected patients aswell as pH1N1 and control specimens
Contributor(s) Kulasinghe AK, Tan CW
Citation(s) 36107637
Submission date Aug 26, 2022
Last update date Oct 28, 2022
Contact name Arutha Kulasinghe
Organization name University of Queensland
Street address 37 kent st
City brisbane
ZIP/Postal code 4102
Country Australia
Platforms (2)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
GPL29487 Illumina NovaSeq 6000 (blank sample)
Samples (50)
GSM6509649 No Template Control 1
GSM6509650 SARS_CoV2_1
GSM6509651 SARS_CoV2_2
BioProject PRJNA874043

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE212119_RAW.tar 1.4 Mb (http)(custom) TAR (of DCC)
GSE212119_TMA1_QC.csv.gz 227.8 Kb (ftp)(http) CSV
GSE212119_TMA1_initial.csv.gz 223.3 Kb (ftp)(http) CSV
GSE212119_TMA2_QC.csv.gz 248.1 Kb (ftp)(http) CSV
GSE212119_TMA2_initial.csv.gz 247.0 Kb (ftp)(http) CSV
GSE212119_normalisedCountMatrix.csv.gz 743.7 Kb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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