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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jul 30, 2010 |
| Title |
DNMT3B7 disrupts embryonic development and accelerates lymphomagenesis |
| Organism |
Mus musculus |
| Experiment type |
Methylation profiling by genome tiling array
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| Summary |
Cancer cells have an altered distribution of DNA methylation and express aberrant DNA methyltransferase 3B transcripts, which encode truncated proteins. To test if a truncated DNMT3B isoform disrupts DNA methylation in vivo, we constructed transgenic mice expressing DNMT3B7, a common truncated DNMT3B isoform in cancer cells. DNMT3B7 transgenic mice exhibit altered embryonic development, including lymphopenia, craniofacial abnormalities, and cardiac defects, similar to Dnmt3b-deficient animals, but rarely develop cancer. However, DNMT3B7 expression increases the frequency of mediastinal lymphomas in Eμ−myc animals. Eμ-myc/DNMT3B7 lymphomas have more chromosomal rearrangements, increased global methylation levels, and more locus-specific perturbations in DNA methylation patterns compared to Eμ-myc lymphomas. Our results demonstrate that a truncated DNMT3B protein can alter tumorigenesis, suggesting a similar role in human tumors.
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| Overall design |
Direct comparison of DNA methylation in lymphoma samples from Eu-Myc vs Eu-Myc/Dnmt3b7 mice.
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| Contributor(s) |
Godley L, Figueroa ME, Melnick A |
| Citation(s) |
20587527 |
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| Submission date |
Apr 08, 2010 |
| Last update date |
Mar 22, 2012 |
| Contact name |
Maria Eugenia Figueroa |
| E-mail(s) |
mef162@miami.edu
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| Organization name |
University of Miiami
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| Department |
Human Genetics
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| Lab |
Maria Figueroa
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| Street address |
1501 NW 10th Ave, BRB 709A, Locator code C227
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| City |
Miami |
| State/province |
FL |
| ZIP/Postal code |
33136 |
| Country |
USA |
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| Platforms (1) |
| GPL10283 |
Nimblegen Mouse 25K MM5_HELP_Promoter array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA126263 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE21273_RAW.tar |
92.3 Mb |
(http)(custom) |
TAR (of PAIR) |
| Processed data included within Sample table |
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