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Status |
Public on Sep 13, 2022 |
Title |
Comparative Study of the Expression Profiles of miRNAs of Milk-Derived Exosomes of Yak and Jeryak |
Organisms |
Bos grunniens; Bos grunniens x Bos taurus |
Experiment type |
Non-coding RNA profiling by high throughput sequencing
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Summary |
The Jeryak is the hybrid offspring of yaks and Jersey cattle and exhibit improved milk and meat yields. Biomolecules carried within milk exosomes are important for cell growth, development, immune regulation, and various pathophysiological processes. Previous studies showed that miRNAs regulate mammary gland development, lactation, and milk quality. This study explored the relationship between milk exosomes miRNAs and lactation performance. A comparison of the milk content showed that yak milk was of a better quality compared to Jeryak milk (casein, fat, TS, SNF, lactose). Milk collected in December was superior to that collected in June for both yak and Jeryak, except for lactose concentrations. Exosomes were extracted by density gradient centrifugation and miRNA expression profiles in milk exosomes from three yaks and three Jeryaks collected in June and December were detected by small RNA sequencing. In all, 22, 120, 78, and 62 differentially expressed miRNAs (DEMs) were identified in Jun_ JY vs. Jun_ Y (P1: Jeryak in June vs. Yak in June), Jun_ JY vs. Dec_ JY (P2: Jeryak in June vs. Jeryak in December), Dec_ JY vs. Dec_ Y (P3: Jeryak in December vs. Yak in December), and Jun_ Y vs. Dec_ Y (P4: Yak in June vs. Yak in December) groups. These DEMs were enriched in functions and signaling pathways related to lactation performance. In conclusion, these findings are a reference tool to study the molecular basis of lactation performance.
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Overall design |
miRNA sequencing of milk exosomes of yak and Jeryak in June and December
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Contributor(s) |
Ren W, Yan P, Liang C |
Citation(s) |
36428417 |
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Submission date |
Sep 08, 2022 |
Last update date |
Dec 22, 2022 |
Contact name |
Xingdong Wang |
E-mail(s) |
wxd17339929758@126.com
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Phone |
17339929758
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Organization name |
CAAS: Chinese Academy of Agricultural Sciences
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Street address |
No.335 Jiangouyan, Qilihe District
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City |
Lanzhou |
State/province |
未选择 |
ZIP/Postal code |
730050 |
Country |
China |
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Platforms (2) |
GPL30409 |
Illumina NovaSeq 6000 (Bos grunniens) |
GPL32644 |
Illumina NovaSeq 6000 (Bos grunniens x Bos taurus) |
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Samples (12)
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Relations |
BioProject |
PRJNA878496 |
Supplementary file |
Size |
Download |
File type/resource |
GSE212938_Processed_data_files.xlsx |
248.2 Kb |
(ftp)(http) |
XLSX |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
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