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| Status |
Public on Jun 30, 2011 |
| Title |
Genome-wide methylation analysis of AOM/DSS-induced colon tumors |
| Organism |
Mus musculus |
| Experiment type |
Methylation profiling by genome tiling array
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| Summary |
Genome-wide methylation analysis was performed by methylated DNA immunoprecipitation (MeDIP)-CpG island (CGI) microarray analysis to identify candidate CGIs specifically methylated in mouse colon tumors associated with colitis. We sucessfully identified 23 candidate CGIs methylated in tumors.
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| Overall design |
Two samples were analyzed by MeDIP-CGI microarray. One is a pool of two AOM/DSS-induced colon tumors in BALB/c mice and another is a pool of two normal colonic epithelial cell samples obtained from untreated BALB/c mice by the crypt isolation technique. The pool of normal colonic epithelial cell samples was used as reference. Dye-swaps were not perfromed. The methylation statuses of CGIs identified by microarray were confirmed by another method, methylation-specific PCR.
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| Contributor(s) |
Katsurano M, Niwa T, Yamashita S, Ushijima T |
| Citation(s) |
21685942 |
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| Submission date |
Apr 14, 2010 |
| Last update date |
Mar 22, 2012 |
| Contact name |
Satoshi Yamashita |
| E-mail(s) |
syamashi@maebashi-it.ac.jp
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| Organization name |
National Cancer Center Research Institute
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| Street address |
Tsukiji 5-1-1
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| City |
Chuo-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
104-0045 |
| Country |
Japan |
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| Platforms (1) |
| GPL10324 |
Agilent-015279 Mouse CpG Island ChIP-on-Chip Microarray 2x105K (Probe Name version) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA126163 |
