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Series GSE21333 Query DataSets for GSE21333
Status Public on Jun 30, 2011
Title Genome-wide methylation analysis of AOM/DSS-induced colon tumors
Organism Mus musculus
Experiment type Methylation profiling by genome tiling array
Summary Genome-wide methylation analysis was performed by methylated DNA immunoprecipitation (MeDIP)-CpG island (CGI) microarray analysis to identify candidate CGIs specifically methylated in mouse colon tumors associated with colitis. We sucessfully identified 23 candidate CGIs methylated in tumors.
Overall design Two samples were analyzed by MeDIP-CGI microarray. One is a pool of two AOM/DSS-induced colon tumors in BALB/c mice and another is a pool of two normal colonic epithelial cell samples obtained from untreated BALB/c mice by the crypt isolation technique. The pool of normal colonic epithelial cell samples was used as reference. Dye-swaps were not perfromed. The methylation statuses of CGIs identified by microarray were confirmed by another method, methylation-specific PCR.
Contributor(s) Katsurano M, Niwa T, Yamashita S, Ushijima T
Citation(s) 21685942
Submission date Apr 14, 2010
Last update date Mar 22, 2012
Contact name Satoshi Yamashita
Organization name National Cancer Center Research Institute
Street address Tsukiji 5-1-1
City Chuo-ku
State/province Tokyo
ZIP/Postal code 104-0045
Country Japan
Platforms (1)
GPL10324 Agilent-015279 Mouse CpG Island ChIP-on-Chip Microarray 2x105K (Probe Name version)
Samples (2)
GSM533093 AOM/DSS-induced colon tumor
GSM533094 normal colonic epithelial cell sample
BioProject PRJNA126163

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE21333_RAW.tar 61.9 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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