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Series GSE213617 Query DataSets for GSE213617
Status Public on Apr 22, 2024
Title Identification and removal of unexpected proliferative off-target cells emerging after implantation of iPSC-derived pancreatic islet cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The differentiation of pancreatic endocrine cells from human pluripotent stem cells (PSC) has been thoroughly investigated for their application in cell therapy against diabetes. However, there is little information available on the long-term safety of these differentiated cells. Here we show that implantation of induced PSC-derived pancreatic islet cells (iPIC) results in the appearance of unexpected proliferative off-target cells via in vivo maturation. Since the unexpected cells have characteristics of both mesenchymal stem cells (MSC) and uterine tissue, we named these cells proliferative MSC and uterus like cells (PMUC). To detect PMUC without implantation, we developed four-week-extended culture system and found that PMUC can be reduced by compound treatment, especially a taxane Docetaxel. Indeed, when we implanted Docetaxel-treated iPIC, the appearance of PMUC disappeared. The present study provides useful insights into identifying and resolving safety issues which is particularly important in the field of cell-based medicine using PSC.
Overall design Single-cell mRNA sequencing of cadaveric human islet, and pluripotent stem cells differentiating in vitro towards pancreatic islet like cells.
Contributor(s) Hiyoshi H, Sakuma K, Asano S, Matsumoto H, Ito R, Toyoda T
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Submission date Sep 19, 2022
Last update date Apr 23, 2024
Contact name Hideyuki Hiyoshi
Organization name Takeda Pharmaceutical Company Limited
Street address 26-1, Muraoka-Higashi 2-Chome
City Fujisawa
State/province Kanagawa
ZIP/Postal code 251-0012
Country Japan
Platforms (1)
GPL20795 HiSeq X Ten (Homo sapiens)
Samples (7)
GSM6589896 Vitro s6-iPIC without PD-166866
GSM6589897 Vitro s6-iPIC
GSM6589898 Human islet tissue
BioProject PRJNA881936

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Supplementary file Size Download File type/resource
GSE213617_RAW.tar 257.1 Mb (http)(custom) TAR (of MTX, TSV)
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Raw data are available in SRA
Processed data provided as supplementary file

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