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| Status |
Public on Feb 02, 2023 |
| Title |
Three-dimensional chromatin re-organization during muscle stem cell aging [Hi-C] |
| Organism |
Mus musculus |
| Experiment type |
Other
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| Summary |
Age-related skeletal muscle atrophy or sarcopenia is a significant societal problem that is becoming amplified as the world’s population continues to increase. A critical contributor to sarcopenia is the loss in the number and function of muscle stem cells, which maintain tissue homeostasis and regenerate damage. The molecular mechanisms that govern muscle stem cell aging encompass changes across multiple regulatory layers and are integrated by the three-dimensional organization of the genome. To quantitatively understand how hierarchical chromatin architecture changes during muscle stem cell aging, we generated 3D chromatin conformation maps (Hi-C) and integrated these datasets with multi-omic (chromatin accessibility and transcriptome) profiles from bulk populations and single cells. We observed that muscle stem cells display static behavior at global scales of chromatin organization during aging and extensive rewiring of local contacts at finer scales that were associated with variations in transcription factor binding and aberrant gene expression. These data provide insights into genome topology as a regulator of molecular function in stem cell aging.
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| Overall design |
Age-matched Hi-C and single-cell ATAC-seq (scATAC-seq) datasets from young (3 months) and aged (24-29 months) mice. Hi-C datasets were generated from muscle stem cells that were FACS-isolated from skeletal hindlimb muscles. scATAC-seq datasets were generated from single nuclei collected from skeletal hindlimb muscles.
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| Contributor(s) |
Yang BA, Aguilar C |
| Citation(s) |
36727578 |
| |
| Submission date |
Sep 23, 2022 |
| Last update date |
Feb 03, 2023 |
| Contact name |
Susanna Rosi |
| E-mail(s) |
srosi@altoslabs.com
|
| Organization name |
Altos labs
|
| Lab |
Rosi Lab
|
| Street address |
2200 Island Dr
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94112 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE214047 |
Three-dimensional chromatin re-organization during muscle stem cell aging |
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| Relations |
| BioProject |
PRJNA883692 |
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