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Series GSE214304 Query DataSets for GSE214304
Status Public on Oct 02, 2022
Title Nascent transcription upon interferon-α2 stimulation on human and rhesus macaque lymphoblastoid cell lines
Organisms Macaca mulatta; Homo sapiens
Experiment type Other
Summary Changes in gene transcription regulation are necessary for species to respond to changes in their environment. In particular, host-pathogen dynamics have been observed to entail rapid evolution of genes involved in the host innate immune system. Cytokines such as type I interferon alpha and beta trigger an antiviral cellular state controlled by members of the transcription factor families STAT and IRF. However, it remains poorly understood how gene transcription regulation has been rewired through evolutionary time to achieve species-specific interferon-controlled changes in gene expression. We generated nascent transcription (PRO-seq) datasets to determine the initial transcriptional response on lymphoblastoid cell lines derived from homo sapiens and macaca mulatta upon stimulation with interferon alpha2 to assess species-specific changes in gene regulation.
Overall design We used lymphoblastoid cell lines derived from two individuals (one male and one female) from homo sapiens and from macaca mulatta; a single replicate per individual, and therefore two samples per species per treatment. PRO-seq datasets were obtained from each individual cell line treated for 1 hour with either BSA (negative control), homo sapiens IFN alpha2, or rhesus macaque IFN alpha2.
Contributor(s) Ramirez D, Dowell RD
Citation(s) 37885027
Submission date Sep 27, 2022
Last update date Nov 13, 2023
Contact name Robin D. Dowell
Phone 303 492 8204
Organization name University of Colorado Boulder
Department Molecular, Cellular and Developmental Biology
Lab Dowell lab
Street address 596 UCB
City Boulder
State/province CO
ZIP/Postal code 80309
Country USA
Platforms (2)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
GPL21120 Illumina NextSeq 500 (Macaca mulatta)
Samples (12)
GSM6603472 PRO-seq, Human LCL female, BSA, 1 hour, rep 1
GSM6603473 PRO-seq, Human LCL male, BSA, 1 hour, rep 1
GSM6603474 PRO-seq, Human LCL female, hsIFNa2, 1 hour, rep 1
BioProject PRJNA884834

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Supplementary file Size Download File type/resource
GSE214304_RAW.tar 2.0 Gb (http)(custom) TAR (of BED, BW)
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Raw data are available in SRA
Processed data provided as supplementary file

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