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Series GSE214685 Query DataSets for GSE214685
Status Public on Oct 07, 2022
Title NSUN2-mediated 5-methylcytosine enhances purine metabolism through ALYREF in retinoblastoma
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary 5-methylcytosine (m5C) RNA modification installed by methyltransferase NSUN2 regulates gene expression and cell fate. Retinoblastoma (RB) is the most common primary intraocular tumor in children. However, the functional role of m5C modification in retinoblastoma remains unclear. Here, we show that 5-methylcytosine significantly promotes the progression of retinoblastoma. Retinoblastoma samples showed increased m5C levels, indicating a poor prognosis. Changes in global m5C modification were highly associated with tumor progression in vitro and in vivo. Mechanistically, NSUN2 stabilized the methylated PFAS mRNA, a tumor promoter in retinoblastoma. Our work uncovers a critical function for m5C methylation in retinoblastoma and provides additional insight into the understanding of m5C modification.
Overall design RNA-seq and MeRIP-seq for retinoblastoma cell lines (Y79) and one retina pigment epithelium cell line (ARPE-19)
Contributor(s) Zuo S, Li L, Chai P, Lu L, Fan X
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Submission date Oct 03, 2022
Last update date Oct 11, 2022
Contact name Sipeng Zuo
Organization name Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine
Street address No. 12, Lane 833, Zhizaoju Road, Huangpu District
City Shanghai
State/province Shanghai
ZIP/Postal code 200023
Country China
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (14)
GSM6614251 RPE-1.Input
GSM6614252 RPE-2.Input
GSM6614253 Y79-1.Input
BioProject PRJNA886684

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE214685_Differentially_Expressed_genes.xlsx 3.1 Mb (ftp)(http) XLSX
GSE214685_Methylated_RNA_sites.mRNA.xlsx 591.8 Kb (ftp)(http) XLSX
GSE214685_Sh-vs-Ctrl-all.gene.xls.gz 3.9 Mb (ftp)(http) XLS
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Raw data are available in SRA
Processed data are available on Series record

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