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| Status |
Public on Oct 07, 2022 |
| Title |
NSUN2-mediated 5-methylcytosine enhances purine metabolism through ALYREF in retinoblastoma |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
5-methylcytosine (m5C) RNA modification installed by methyltransferase NSUN2 regulates gene expression and cell fate. Retinoblastoma (RB) is the most common primary intraocular tumor in children. However, the functional role of m5C modification in retinoblastoma remains unclear. Here, we show that 5-methylcytosine significantly promotes the progression of retinoblastoma. Retinoblastoma samples showed increased m5C levels, indicating a poor prognosis. Changes in global m5C modification were highly associated with tumor progression in vitro and in vivo. Mechanistically, NSUN2 stabilized the methylated PFAS mRNA, a tumor promoter in retinoblastoma. Our work uncovers a critical function for m5C methylation in retinoblastoma and provides additional insight into the understanding of m5C modification.
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| Overall design |
RNA-seq and MeRIP-seq for retinoblastoma cell lines (Y79) and one retina pigment epithelium cell line (ARPE-19)
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| Contributor(s) |
Zuo S, Li L, Chai P, Lu L, Fan X |
| Citation missing |
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| |
| Submission date |
Oct 03, 2022 |
| Last update date |
Oct 11, 2022 |
| Contact name |
Sipeng Zuo |
| E-mail(s) |
Zsp2019@sjtu.edu.cn
|
| Organization name |
Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine
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| Street address |
No. 12, Lane 833, Zhizaoju Road, Huangpu District
|
| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
200023 |
| Country |
China |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA886684 |
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