|
| Status |
Public on Oct 11, 2022 |
| Title |
Sen1 and Rrm3 ensure permissive topological conditions for replication termination [ChIP-seq] |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Replication forks terminate at TERs and telomeres. Forks that converge or encounter transcription generate topological stress. Combining genetic, genomic and imaging approaches we found that Rrm3hPif1 and Sen1hSenataxin helicases assist termination at TERs, Sen1 at telomeres. rrm3 and sen1 are synthetic lethal, fail to terminate replication exhibiting lagging chromosomes and fragility at TERs and telomeres. sen1 rrm3 build up RNA-DNA hybrids at TERs, sen1 accumulates RNPII at TERs and telomeres. Double mutants exhibit X-shaped gapped or reversed converging forks. Rrm3 and Sen1 restrain Top1 and Top2 activities, preventing toxic accumulation of positive supercoil at TERs and telomeres. We suggest that Rrm3 and Sen1 coordinate the activities of fork-associated Top1 and Top2 with those of gene loop-associated Top1 and Top2 by preventing DNA and RNA polymerases slowing down when forks encounter transcription head-on or codirectionally, respectively. Hence Rrm3 and Sen1 are essential to generate permissive topological conditions for replication termination.
|
| |
|
| Overall design |
RNA Pol II_Rpb1-ChIP-seq (S-phase) and bTMP-seq
|
| |
|
| Contributor(s) |
Choudhary R, Adhil M, Foiani M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Oct 06, 2022 |
| Last update date |
Oct 13, 2022 |
| Contact name |
Mohamood Adhil Mohammed Iqbal |
| E-mail(s) |
adhil.md@gmail.com
|
| Organization name |
IFOM - The FIRC Institute of Molecular Oncology
|
| Street address |
Via Adamello 16
|
| City |
Milano |
| State/province |
Lombardia |
| ZIP/Postal code |
20139 |
| Country |
Italy |
| |
|
| Platforms (1) |
| GPL26302 |
NextSeq 550 (Saccharomyces cerevisiae) |
|
| Samples (48)
|
|
| This SubSeries is part of SuperSeries: |
| GSE214930 |
Sen1 and Rrm3 ensure permissive topological conditions for replication termination |
|
| Relations |
| BioProject |
PRJNA887754 |
Less...
More...